Network analysis of icb-1 gene function in human breast cancer cells

Journal of Cellular Biochemistry
Oliver TreeckOlaf Ortmann

Abstract

Icb-1 is a human gene previously described by our group to exert important functions in cancer cells of different origin. We now performed microarray-based gene expression profiling with subsequent network modeling to further elucidate the role of icb-1 in breast cancer cells. Analyzing the effect of icb-1 knockdown on the transcriptome of MCF-7 cells, we found 151 differentially expressed genes exhibiting more than twofold changes, 97 of which were up- and 54 downregulated. Most of the upregulated genes were cancer-related genes associated with poor prognosis, invasion and metastasis, building an oncogenic network of TNF target genes. On the other hand, network analysis identified the downregulated genes to be primarily involved in interferon signaling and cellular apoptosis. Confirming these network data, we observed that cells with reduced levels of icb-1 exhibited an impaired response to the apoptosis inducers tamoxifen, staurosporine, actinomycin, and camptothecin. The data of this study suggest that icb-1 might exert a tumor-suppressor function in breast cancer and that its loss might confer relative resistance of breast cancer cells to apoptotic drugs.

References

Nov 1, 1994·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J Welsh
Feb 15, 2001·Nature Cell Biology·P ListonR G Korneluk
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 16, 2002·The Journal of Biological Chemistry·Heather N AndrewsD Paul Harkin
Dec 22, 2004·The Journal of Investigative Dermatology·Kin Cheung P NgGang Li
Jul 20, 2005·Trends in Immunology·Martina Schröder, Andrew G Bowie
Jan 5, 2007·BMC Cancer·Rosalyn D BlumenthalDavid M Goldenberg
Jul 26, 2007·BMC Cancer·Marc TischkowitzWilliam D Foulkes
Nov 21, 2007·Differentiation; Research in Biological Diversity·Michal GilonUri Gat
Jan 22, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Julia BollmannOliver Treeck
Mar 12, 2008·Apoptosis : an International Journal on Programmed Cell Death·Shaun Rosebeck, Douglas W Leaman
May 24, 2008·Advances in Experimental Medicine and Biology·Manuel Valladares AyerbesLuis Antón Aparicio
Jun 19, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Xin-Rong YangZhao-You Tang
Jul 11, 2008·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Joan S Lewis-WambiV Craig Jordan
Sep 30, 2008·Nature Cell Biology·Sachie HiratsukaYoshiro Maru
Jan 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christophe RichezIan R Rifkin
Feb 18, 2009·Journal of Cancer Research and Clinical Oncology·Manuel Valladares-AyerbesLuis M Antón-Aparicio
Mar 25, 2009·Cancer Investigation·Anette SpringwaldOliver Treeck
Apr 10, 2009·International Journal of Cancer. Journal International Du Cancer·Shui Ping TuBenjamin Chun-Yu Wong
Nov 28, 2009·Endocrine-related Cancer·Anna KonwisorzOliver Treeck

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Citations

Mar 12, 2013·Cancer Letters·Oliver TreeckOlaf Ortmann

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