Network-based proteomic approaches reveal the neurodegenerative, neuroprotective and pain-related mechanisms involved after retrograde axonal damage

Scientific Reports
Caty CasasPatrick Aloy

Abstract

Neurodegenerative processes are preceded by neuronal dysfunction and synaptic disconnection. Disconnection between spinal motoneuron (MN) soma and synaptic target leads either to a retrograde degenerative process or to a regenerative reaction, depending injury proximity among other factors. Distinguished key events associated with one or other processes may give some clues towards new therapeutical approaches based on boosting endogenous neuroprotective mechanisms. Root mechanical traction leads to retrograde MN degeneration, but share common initial molecular mechanisms with a regenerative process triggered by distal axotomy and suture. By 7 days post-injury, key molecular events starts to diverge and sign apart each destiny. We used comparative unbiased proteomics to define these signatures, coupled to a novel network-based analysis to get biological meaning. The procedure implicated the previous generation of combined topological information from manual curated 19 associated biological processes to be contrasted with the proteomic list using gene enrichment analysis tools. The novel and unexpected results suggested that motoneurodegeneration is better explained mainly by the concomitant triggering of anoikis, anti-apoptotic ...Continue Reading

References

Sep 26, 2000·Molecular and Cellular Biology·S J CharetteJ Landry
Sep 7, 2001·Current Opinion in Cell Biology·S M Frisch, R A Screaton
Jul 12, 2002·Nature·Paola ScaffidiMarco E Bianchi
Oct 2, 2002·Nature Biotechnology·Gary D Bader, Christopher W V Hogue
Dec 19, 2003·Nucleic Acids Research·Lukasz SalwinskiDavid Eisenberg
Dec 31, 2003·Molecular and Cellular Neurosciences·Woong Sun, Ronald W Oppenheim
Feb 28, 2004·Journal of Neurotrauma·Antoni Valero-CabréWolfram F Neiss
Apr 16, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniel PerreletAnn C Kato
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Mar 24, 2005·Journal of Cellular and Molecular Medicine·Gang SongShideng Bao
Oct 26, 2005·Cell Death and Differentiation·A P Gilmore
Nov 3, 2006·Brain Research Reviews·Gennadij Raivich, Milan Makwana
Feb 6, 2007·Progress in Neurobiology·John R MoffettAryan M A Namboodiri
Mar 8, 2007·Annual Review of Neuroscience·Zu-Lin ChenSidney Strickland
May 8, 2007·Trends in Cell Biology·Karim Bensaad, Karen H Vousden
Sep 8, 2007·The European Journal of Neuroscience·Ok-hee ParkWoong Sun
Apr 4, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Su Yeon ShimMinh Dang Nguyen
May 13, 2008·Trends in Biochemical Sciences·Andrew Chatr-AryamontriGianni Cesareni
Jun 17, 2008·Bioinformatics·Johannes GollPeter Uetz
Jun 25, 2008·Current Opinion in Cell Biology·Rebecca Lock, Jayanta Debnath
Sep 4, 2008·Molecular Systems Biology·David J LynnFiona S L Brinkman
Nov 8, 2008·Nucleic Acids Research·T S Keshava PrasadAkhilesh Pandey
Apr 1, 2009·Journal of Neurotrauma·Clara PenasXavier Navarro
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Oct 24, 2009·FEBS Letters·Tobias MaierLuis Serrano
Oct 29, 2009·Current Molecular Medicine·R A StetlerJ Chen
Nov 10, 2009·Nucleic Acids Research·Arnaud CeolGianni Cesareni
Jan 19, 2010·Experimental Neurology·Kevin K ParkZhigang He
Jul 9, 2010·The Journal of Investigative Dermatology·Roberta LottiCarlo Pincelli
Aug 21, 2010·The European Journal of Neuroscience·Shinji MatsumuraSeiji Ito
Sep 21, 2010·Nucleic Acids Research·Emilie ChautardSylvie Ricard-Blum
Oct 21, 2010·Physiological Reviews·Brinda RavikumarDavid C Rubinsztein
Nov 4, 2010·Nucleic Acids Research·Damian SzklarczykChristian von Mering
Nov 13, 2010·Nucleic Acids Research·Chris StarkMike Tyers

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Citations

Nov 10, 2015·PM & R : the Journal of Injury, Function, and Rehabilitation·Dae Hyun KimYoon Ghil Park
Mar 21, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J C Gomez-VerjanR Reyes-Chilpa
Feb 1, 2018·Scientific Reports·David Romeo-GuitartCaty Casas
Sep 13, 2020·Journal of Molecular Neuroscience : MN·Valentina DzreyanAnatoly Uzdensky
Jul 17, 2020·Computational and Structural Biotechnology Journal·George MinadakisGeorge M Spyrou
Feb 14, 2021·Cells·Sara Marmolejo-Martínez-ArteseroDavid Romeo-Guitart
Aug 28, 2021·Biomedicines·Tatiana Leiva-RodríguezCaty Casas

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Methods Mentioned

BETA
pharmacotherapy
dissection
acetylation
affinity purification

Software Mentioned

Gene Snap
R
Mascot
Gene Tools Genome apparatus
phenoTest ( R package
Bioconductor library
Anoikis
Proteome Discoverer
DAVID
GSEA

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