Jun 2, 2020

Network Pharmacology-Based Approach Uncovers the Mechanism of GuanXinNing Tablet for Treating Thrombus by MAPKs Signal Pathway

Frontiers in Pharmacology
Mu-Lan WangMin-Li Chen

Abstract

GuanXinNing tablet (GXNT), a traditional Chinese patent medicine, has been found to have remarkable antithrombotic effects and can effectively inhibit pro-thrombotic factors in previous studies. However, the mechanism of its antithrombotic effects remains little known. In this study, we first determined and identified the sources of each main compound in GXNT using liquid chromatography-mass spectrometry (LC-MS). Through the approach of network pharmacology, we predicted the action targets of the active components, mapped the target genes related to thrombus, and obtained potential antithrombotic targets for active ingredients. We then performed gene ontology (GO) enrichment analyses and KEGG signaling pathway analyses for the action targets, and constructed networks of active component-target and active component-target-pathway for GXNT. Additionally, we evaluated the pharmacodynamic effects of GXNT on thrombus using the rat thrombus model induced by FeCl3, observed the effects of antiplatelet aggregation via platelet assay, and further verified the results predicted by network pharmacology via Western blot. In total, 14 active ingredients were identified in GXNT, and 83 action targets were predicted, 17 of which are antithrom...Continue Reading

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Mentioned in this Paper

Signal Pathways
Oxidative Stress
Pharmacodynamics
Regulation of Blood Vessel Endothelial Cell Migration
Keto Acids
GORAB
SERPINE1
Protein Aggregation, Pathological
Platelet Aggregation
Liquid Chromatography Mass Spectrometry

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