Network reduction in metabolic pathway analysis: elucidation of the key pathways involved in the photoautotrophic growth of the green alga Chlamydomonas reinhardtii

Metabolic Engineering
Marco RügenGuillaume Cogne

Abstract

Metabolic pathway analysis aims at discovering and analyzing meaningful routes and their interactions in metabolic networks. A major difficulty in applying this technique lies in the decomposition of metabolic flux distributions into elementary mode or extreme pathway activity patterns, which in general is not unique. We propose a network reduction approach based on the decomposition of a set of flux vectors representing adaptive microbial metabolic behavior in bioreactors into a minimal set of shared pathways. Several optimality criteria from the literature were compared in order to select the most appropriate objective function. We further analyze photoautotrophic metabolism of the green alga Chlamydomonas reinhardtii growing in a photobioreactor under maximal growth rate conditions. Key pathways involved in its adaptive metabolic response to changes in light influx are identified and discussed using an energetic approach.

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Citations

Apr 2, 2015·Current Opinion in Biotechnology·Caroline BaroukhJean-Philippe Steyer
Jul 23, 2015·Applied Microbiology and Biotechnology·Bassem Ben YahiaElmar Heinzle
Aug 24, 2017·Journal of Experimental Botany·Fiona Wanjiku MoejesOliver Ebenhöh
Sep 12, 2018·Biotechnology for Biofuels·Juan D Tibocha-BonillaKarsten Zengler
Jul 9, 2020·Progress in Lipid Research·Sandra M CorreaYariv Brotman

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