Neural cell adhesion molecule (NCAM) isoform expression is associated with neuroblastoma differentiation status

Pediatric Blood & Cancer
Cynthia WinterJohn M Maris

Abstract

NCAM is a member of the immunoglobulin superfamily of cell adhesion molecules. While highly expressed on neuroblastoma cells, the relative contribution of the three major NCAM isoforms (120, 140, and 180 kDa) to neuroblastoma biology has not been investigated. NCAM protein expression was measured in a neuroblastic tumor tissue microarray (N = 185) by immunohistochemistry. Relative expression of NCAM mRNA isoforms was measured in a panel of 24 human neuroblastomas and compared to fetal and adult human brain using real-time quantitative PCR and Western blot analysis. Associations with clinical and tumor biological co-variates were performed. NCAM protein was detected on all neuroblastic tumors and was highly expressed in all but 7/167 cases. The mRNA species predicted to encode the 120 kDa protein species was the most abundant isoform in adult brain, ganglioneuromas and ganglioneuroblastomas (P = 0.0007), but the mRNA predicted to encode the 180 kDa species was predominant in neuroblastomas (P = 0.043). Microdissected ganglion and neuroblast cells from human primary tumors confirmed these findings. Ganglioneuromas and ganglioneuroblastomas express the adhesive 120 kDa NCAM isoform, while neuroblastomas preferentially express the ...Continue Reading

References

Jul 1, 1991·Journal of Clinical Pathology·E PhimisterK Patel
Jul 1, 1991·The Journal of Cell Biology·A AchesonU Rutishauser
Jan 1, 1990·Journal of Molecular Neuroscience : MN·J J HemperlyR A Reid
Jul 15, 1987·International Journal of Cancer. Journal International Du Cancer·M LipinskiC Goridis
Feb 15, 1994·Journal of Neuroscience Research·R ProbstmeierJ Schneider-Schaulies
Feb 1, 1996·Leukemia & Lymphoma·U KaiserM Oldenburg
Mar 28, 1997·The Journal of Biological Chemistry·H E BeggsP F Maness
Oct 27, 1997·American Journal of Surgery·J RoeslerE H Livingston
Jan 1, 1997·Annual Review of Cell and Developmental Biology·F S Walsh, P Doherty
Oct 20, 1998·The American Journal of Surgical Pathology·S LantuejoulE Brambilla
Jan 21, 2000·Brain Research. Molecular Brain Research·C S RicardM R Hernandez
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
May 4, 2000·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·E VuralE Hanna
Aug 5, 2003·Molecular and Cellular Biology·Ralph SeidenfadenHerbert Hildebrandt
Jul 16, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Athanasios K PetridisUrs Rutishauser
Nov 25, 2005·The New England Journal of Medicine·Edward F AttiyehUNKNOWN Children's Oncology Group
Jun 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Radhika PeddintiSusan L Cohn

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Citations

Oct 13, 2011·The American Journal of Surgical Pathology·Lisa M SullivanAlexander R Judkins
Mar 28, 2013·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Elaine S ChanLisa M Sullivan
Jul 5, 2008·Neurochemical Research·Kateryna Kolkova
Jun 15, 2011·Journal of Neuropathology and Experimental Neurology·Sriram VennetiAlexander R Judkins
Nov 26, 2015·Cancer Letters·Lucia BorrielloYves A DeClerck
Jun 7, 2014·Frontiers in Oncology·Nora Berois, Eduardo Osinaga
Jun 3, 2017·Cell Death and Differentiation·Alberto DelaidelliPoul H Sorensen
Sep 17, 2011·The American Journal of Surgical Pathology·Sriram VennetiAlexander R Judkins
Aug 28, 2016·Journal of Cell Science·Külli JaakoAlexander Zharkovsky
Nov 14, 2014·Clinical Anatomy : Official Journal of the American Association of Clinical Anatomists & the British Association of Clinical Anatomists·Horia Muresian
Oct 25, 2017·Expert Opinion on Orphan Drugs·Mark A ApplebaumSusan L Cohn
Sep 5, 2014·Cancer Discovery·Jiangbin YeCraig B Thompson
Jul 22, 2017·Molecular Cancer Therapeutics·Ela MarkovskyRonit Satchi-Fainaro
Jun 4, 2021·Journal of Experimental & Clinical Cancer Research : CR·Chiara BrignoleFabio Pastorino

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