Feb 18, 2010

Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency

Proceedings of the National Academy of Sciences of the United States of America
Bao-Yang HuSu-Chun Zhang

Abstract

For the promise of human induced pluripotent stem cells (iPSCs) to be realized, it is necessary to ask if and how efficiently they may be differentiated to functional cells of various lineages. Here, we have directly compared the neural-differentiation capacity of human iPSCs and embryonic stem cells (ESCs). We have shown that human iPSCs use the same transcriptional network to generate neuroepithelia and functionally appropriate neuronal types over the same developmental time course as hESCs in response to the same set of morphogens; however, they do it with significantly reduced efficiency and increased variability. These results were consistent across iPSC lines and independent of the set of reprogramming transgenes used to derive iPSCs as well as the presence or absence of reprogramming transgenes in iPSCs. These findings, which show a need for improving differentiation potency of iPSCs, suggest the possibility of employing human iPSCs in pathological studies, therapeutic screening, and autologous cell transplantation.

Mentioned in this Paper

Flow Cytometry
Pluripotent Stem Cells
Neurons
Enhanced S-Cone Syndrome
Human Induced Pluripotent Stem Cells
Immunocytochemistry
Bone Morphogenetic Proteins
Recombinant Transgenes
Cell Differentiation Process
Neuronal

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