Neural differentiation of mouse embryonic and mesenchymal stem cells in a simple medium containing synthetic serum replacement

Journal of Biotechnology
Masoumeh Fakhr TahaAli Reza Khalatbary

Abstract

Neural differentiation of embryonic and adult stem cells has been reported previously. Several studies have used different proportions of serum or a cocktail of growth and differentiation factors for this purpose. In the present study, we examined neural differentiation of mouse embryonic stem (ES) cells in KoSR-containing media. We also investigated neural differentiation of mouse adipose tissue-derived stem cells (ADSCs) in a medium containing KoSR, a synthetic serum replacement, and compared it with neural differentiation in low-serum condition. Meanwhile, effect of β-ME on neural differentiation was investigated in both conditions. As revealed by RT-PCR and immunocytochemistry analyses, KoSR-containing medium induced neural differentiation of mouse ES cells. Moreover, under the culture conditions we tested, ADSCs were differentiated to neuron-like cells and expressed some neuronal markers. Low concentration of β-ME improved neuron-like differentiation of the ADSCs in the 4% FBS-supplemented medium, while addition of β-ME in KoSR condition decreased neural differentiation. KoSR-containing medium without any additional factor improved generation of neuron-like cells, upregulated the expression of mature neuronal markers and l...Continue Reading

References

Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M M Shen, P Leder
Jan 1, 1992·Cell Motility and the Cytoskeleton·R A Nixon, T B Shea
May 15, 1991·Biochemical and Biophysical Research Communications·D S CramL C Harrison
Jan 1, 1989·Annual Review of Neuroscience·R E ZigmondA R Rittenhouse
Jul 1, 1972·Clinica Chimica Acta; International Journal of Clinical Chemistry·T NagatsuI Nagatsu
Apr 1, 1984·The Journal of Cell Biology·N HirokawaM B Willard
Feb 1, 1993·Journal of Neurochemistry·D L Martin, K Rimvall
Apr 4, 1997·Science·R McKay
Nov 11, 1998·Nature Medicine·P S ErikssonF H Gage
Feb 27, 1999·Experimental Cell Research·M V Wiles, B M Johansson
Oct 16, 1999·Science·E GouldC G Gross
Dec 29, 2000·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·Y C HalvorsenJ M Gimble
Jun 8, 2002·Biochemical and Biophysical Research Communications·Kristine M SaffordHenry E Rice
Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
Jan 14, 2003·Nature Biotechnology·Qi-Long YingAustin Smith
Apr 25, 2003·Plastic and Reconstructive Surgery·Peter H AshjianMarc H Hedrick
Dec 5, 2003·Reproductive Biomedicine Online·Hossein Baharvand, Klaus Ingo Matthaei
Mar 5, 2004·Development·Atsushi KuboGordon Keller
Jun 24, 2004·Journal of Neuroscience Research·Paul LuMark H Tuszynski
Jul 24, 2004·Differentiation; Research in Biological Diversity·Hossein BaharvandDavood Sabour
Jun 1, 2005·Experimental Cell Research·Andrea DickerMikael Rydén
Jun 9, 2005·Biochemical and Biophysical Research Communications·Juri FujimuraHidenori Suzuki
Jul 16, 2005·Journal of Cellular Physiology·Farshid GuilakJeffrey M Gimble
Jul 19, 2005·Annual Review of Neuroscience·Guo-li Ming, Hongjun Song
Dec 13, 2006·International Journal of Cardiology·Masoumeh Fakhr TahaSeyed Javad Mowla
Dec 21, 2006·Differentiation; Research in Biological Diversity·Hongxiu NingChing-Shwun Lin
Mar 7, 2007·The Journal of Veterinary Medical Science·Pang-Yen TsengYong-San Huang

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Citations

Oct 19, 2016·Environmental Toxicology·Mohammad Hadi ZareiMasoumeh Fakhr Taha
Dec 17, 2014·Toxicology Mechanisms and Methods·Mehdi Qasemian LemraskiEmad Jafarzade
Feb 20, 2019·Journal of Cellular Physiology·Ana Patricia Ayala-CuellarKyung-Chul Choi
Jul 5, 2017·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Isha VermaPolani B Seshagiri
Oct 14, 2020·Journal of Molecular Neuroscience : MN·Princy ChoudharySangeeta Singh

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