Neural differentiation of rat adipose-derived stem cells in vitro.

Cellular and Molecular Neurobiology
Chengcheng YingShiwen Li

Abstract

It is reported that adipose-derived stem cells (ADSCs) had multilineage differentiation potential, and could differentiate into neuron-like cells induced by special induction media, which may provide a new idea for restoration of erectile dysfunction (ED) after cavernous nerve injury. The aim of this research was to explore the neuronal differentiation potential of ADSCs in vitro. ADSCs isolated from inguinal adipose tissue of rat were characterized by flow cytometry, and results showed that ADSCs were positive for mesenchymal stem cell markers CD90 and CD44, but negative for hematopoietic stem cell markers. ADSCs maintained self-renewing capacity and could differentiate into adipocytes and neurocytes under special culture condition. In this research, two methods were used to induce ADSCs. In method 1, ADSCs were treated with the preinduction medium including epithelium growth factor, basic fibroblast growth factor, and brain derived neurotrophic factor (BDNF) for 3 days, then with the neurogenic induction medium containing isobutylmethylxanthine, indomethacin, and insulin. While in method 2, BDNF was not used to treat ADSCs. After induction, neuronal differentiation of ADSCs was evaluated. Neuronal markers, glial fibrillary ac...Continue Reading

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Citations

Nov 20, 2012·Cellular and Molecular Neurobiology·Chengcheng YingXinghuan Wang
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Mar 15, 2013·Stem Cells Translational Medicine·Virginie NeirinckxSabine Wislet-Gendebien
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Feb 15, 2019·Neural Regeneration Research·Cheng-Cheng YingXin-Min Zheng

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