Abstract
Primate embryonic stem (ES) cells are capable of indefinite, undifferentiated proliferation and maintain the potential to differentiate to trophoblast and derivatives of embryonic endoderm, mesoderm, and ectoderm. We previously reported that neural differentiation by rhesus ES cells in teratomas includes tissue with a remarkable resemblance to neural tube (Thomson et al. 1995). Here we examine a series of markers including a cell proliferation marker, neurofilament proteins, and glial fibrillary acidic protein (GFAP) in teratomas at 5, 6, 7, 9, and 12 weeks after rhesus ES cell transplantation into muscles of immunodeficient mice. All teratomas examined contained derivatives of all three embryonic germ layers. Neural differentiation included tissues resembling neural tube and embryonic ganglia, as well as individual dispersed neurons, and brain-like gray matter. Tumours of all ages contained neurons and proliferating cells, indicated by staining for neurofilament subunits and Ki67 antigens. Younger tumours contained no or few astrocytes indicated by the absence of GFAP staining, but as these tumours developed, there was an increase in astrocyte differentiation. The results indicate that normal neural differentiation is recapitu...Continue Reading
References
Aug 15, 1991·The Journal of Comparative Neurology·T TohyamaJ Q Trojanowski
Nov 1, 1991·Pediatric Neurology·L A LockmanW Krivit
Apr 16, 1991·Biochimica Et Biophysica Acta·J W ShayH Werbin
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·V M LeeJ Q Trojanowski
Jun 1, 1984·Developmental Biology·P W Andrews
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R S BurnsI J Kopin
Apr 1, 1983·Developmental Biology·P LevittP Rakic
Jan 1, 1984·Journal of Neurochemistry·V M LeeW W Schlaepfer
Sep 9, 1982·Nature·M W McBurneyP J Anderson
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·J A ThomsonJ P Hearn
Aug 15, 1993·The Journal of Comparative Neurology·A T YachnisJ Q Trojanowski
Apr 1, 1995·Developmental Biology·G BainD I Gottlieb
Nov 1, 1993·The Journal of Cell Biology·C SchlüterJ Gerdes
Jan 1, 1993·European Urology·S RoachM F Pera
Apr 10, 1996·Developmental Biology·B A Reynolds, S Weiss
Feb 1, 1996·Experimental Neurology·C N SvendsenS B Dunnett
Sep 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·H C Pant, Veeranna
Aug 1, 1996·Biology of Reproduction·J A ThomsonJ P Hearn
Sep 1, 1996·Trends in Neurosciences·S WeissD van der Kooy
Dec 23, 1996·The Journal of Comparative Neurology·M MiyazonoJ Q Trojanowski
Citations
May 23, 2012·Molecular Neurobiology·Aisha Iman AbdullahTao Sun
Dec 13, 2005·International Journal of Cardiology·Panayiotis M Zavos
Mar 17, 2000·Neuron·C ScharffF Nottebohm
Jun 3, 2009·Antioxidants & Redox Signaling·Kathleen M FitzpatrickMarina E Emborg
May 7, 2004·Biology of Reproduction·Don P WolfLinda Lester
Apr 3, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·K M Grigor, C C Wylie
Jun 8, 2002·Annual Review of Neuroscience·David I Gottlieb
Feb 26, 2013·PloS One·Steven LotzChristopher A Fasano
Jan 10, 2013·Experimental Neurology·Fahad AliRoger A Barker
Jul 24, 2012·Gene·Nestor L Lopez CorralesNadezda Kosyakova
Oct 18, 2011·Neuroscience·M ChafaiE Louiset
Jan 5, 2011·Theriogenology·Catharina De SchauwerAnn Van Soom
Mar 11, 2009·Differentiation; Research in Biological Diversity·Xiao Ling KuaiBruce A Bunnell
Aug 9, 2008·Stem Cells·Anderson Hsien-Cheng HuangAnthony W S Chan
Mar 22, 2008·Stem Cells·Florence WiannyColette Dehay
Nov 30, 2006·Journal of Neuroscience Research·Perrine BarraudAnders Björklund
Jun 6, 2003·Biochemical and Biophysical Research Communications·John D CalhounSteven L Stice
Aug 6, 2005·Molecular and Cellular Neurosciences·Pavel ItsyksonBenjamin E Reubinoff
Sep 24, 1999·Developmental Biology·T MujtabaM S Rao
Jun 18, 2004·Reproductive Biology and Endocrinology : RB&E·Anthony W S Chan
Apr 28, 2004·Developmental Biology·Irene GinisMahendra S Rao
Jul 31, 2008·PloS One·Shinobu UedaTakahiro Ochiya
Jun 7, 2006·Biochemical and Biophysical Research Communications·Yanxia LiuHongkui Deng
Dec 20, 1999·The Journal of Comparative Neurology·R S HartleyV M Lee
Mar 30, 2004·Journal of Neuroscience Research·Kenneth J D LangPeter D Rathjen
Jun 19, 2004·Reviews in the Neurosciences·Susanne NikolausRolf Larisch
Dec 3, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Yasumasa Kuroda, Mari Dezawa
Oct 18, 2003·Blood·Dan S KaufmanJames A Thomson
Nov 20, 2016·Neural Regeneration Research·Li-Juan YeFeng Chen
Sep 16, 2017·Scientific Reports·Eui-Hwan ChoiKeun P Kim
Jan 4, 2019·Tissue Engineering and Regenerative Medicine·Ningsheng RuanYanding Zhang
Feb 1, 2007·Acta Neuropsychiatrica·Michael ValenzuelaPerminder Sachdev
Jul 6, 2019·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Arubala P ReddyNurgul Carkaci-Salli
Feb 26, 2014·Stem Cell Research & Therapy·Zhonggang HouJames A Thomson
May 14, 2003·Stem Cells·Silvia S ChenLeonid Margolis