Neural G0: a quiescent-like state found in neuroepithelial-derived cells and glioma.

Molecular Systems Biology
Samantha A O'ConnorChristopher L Plaisier

Abstract

Single-cell RNA sequencing has emerged as a powerful tool for resolving cellular states associated with normal and maligned developmental processes. Here, we used scRNA-seq to examine the cell cycle states of expanding human neural stem cells (hNSCs). From these data, we constructed a cell cycle classifier that identifies traditional cell cycle phases and a putative quiescent-like state in neuroepithelial-derived cell types during mammalian neurogenesis and in gliomas. The Neural G0 markers are enriched with quiescent NSC genes and other neurodevelopmental markers found in non-dividing neural progenitors. Putative glioblastoma stem-like cells were significantly enriched in the Neural G0 cell population. Neural G0 cell populations and gene expression are significantly associated with less aggressive tumors and extended patient survival for gliomas. Genetic screens to identify modulators of Neural G0 revealed that knockout of genes associated with the Hippo/Yap and p53 pathways diminished Neural G0 in vitro, resulting in faster G1 transit, down-regulation of quiescence-associated markers, and loss of Neural G0 gene expression. Thus, Neural G0 represents a dynamic quiescent-like state found in neuroepithelial-derived cells and gli...Continue Reading

References

Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W E MercerS J Ullrich
May 1, 1995·Trends in Biochemical Sciences·C J Sherr
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·E G Van MeirW K Cavenee
Mar 1, 1994·Molecular and Cellular Biology·H MatsushimeJ Y Kato
Dec 1, 1995·Current Opinion in Cell Biology·A ZetterbergK G Wiman
Sep 1, 1996·Cytometry·Z DarzynkiewiczF Traganos
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Apr 20, 2001·Brain Research. Molecular Brain Research·K YonedaT Kato
Nov 2, 2001·Nature·T ReyaI L Weissman
Jun 12, 2002·Molecular Biology of the Cell·Michael L WhitfieldDavid Botstein
Sep 3, 2002·Cancer Cell·Charles J Sherr, Frank McCormick
Aug 30, 2003·The Journal of Biological Chemistry·Masami SakamotoRyoichiro Kageyama
Oct 11, 2003·Current Opinion in Genetics & Development·Fiona Doetsch
Mar 3, 2004·Nature Reviews. Cancer·P Andrew FutrealMichael R Stratton
Mar 9, 2004·Genes & Development·Daniel P Kelly, Richard C Scarpulla
Mar 11, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Suzana Herculano-Houzel, Roberto Lent
Jun 13, 2006·Cerebral Cortex·Steven M PollardAustin Smith
Dec 22, 2006·Genes & Development·Maria BergslandJonas Muhr
Apr 25, 2007·Molecular and Cellular Biology·Etsuo SusakiKeiichi I Nakayama
Jan 30, 2008·The Journal of Cell Biology·Haifan Lin
Feb 13, 2008·Cell·Asako Sakaue-SawanoAtsushi Miyawaki
Mar 28, 2008·Nature Cell Biology·Guang YaoLingchong You
May 3, 2008·Molecular and Cellular Neurosciences·Yirui SunAustin Smith
Jun 10, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Peter B Dirks
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
May 12, 2009·Cell Stem Cell·Myung Jin SonHoward A Fine
Feb 6, 2010·Genome Biology·Matthew D YoungAlicia Oshlack
Sep 28, 2010·Nature Neuroscience·Charlotte E ScottJames Briscoe
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Jun 15, 2011·Journal of Neuropathology and Experimental Neurology·Brent A OrrCharles G Eberhart
Sep 14, 2011·Journal of Neuro-oncology·Li LiYanchun Deng
Jan 29, 2013·Science Signaling·Jane I LinKieran F Harvey
May 9, 2013·Genes & Development·Christopher G HubertPatrick J Paddison

❮ Previous
Next ❯

Software Mentioned

learn
ssGSEA
GeneMANIA
GSEA
Python
R
HTSeq
ACTINN
NIS
ccAF

Related Concepts

Related Feeds

Cancer Stem Cells in Glioblastoma

Glioblastoma is the most common and aggressive type of brain tumor. It contains a population of tumor initiating stem cell-like cells known as cancer stem cells. Investigations are ongoing into these cancer stem cells found in these solid tumors which are highly resistance to treatment. Here is the latest research on cancer stem cells in glioblastoma.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

CZI Human Cell Atlas Seed Network

The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.

Related Papers

BioRxiv : the Preprint Server for Biology
Heather FeldmanChristopher Plaisier
BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology
S Cooper
BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology
D A Gilbert
BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology
P Fantes
© 2021 Meta ULC. All rights reserved