Neural progenitors populate the cerebrospinal fluid of preterm patients with hydrocephalus

The Journal of Pediatrics
Richard C KruegerYi E Sun

Abstract

To evaluate cerebrospinal fluid (CSF) of preterm patients with hydrocephalus for neural progenitors. This report describes a prospective study of CSF obtained from preterm infants, either with progressive posthemorrhagic hydrocephalus (PPHH) or without known intercranial pathology. Cells recovered by centrifugation were analyzed by reverse transcriptase-polymerase chain reaction or by immunocytometry. Alternatively, cells were cultured by using methods permissive to neural progenitor growth and analyzed by immunocytochemistry and Western blotting. Human CSF cells were obtained from 20 preterm infants at approximately 27 weeks estimated gestational age (15 infants with PPHH, 5 control infants). The number of these cells removed over time from patients with PPHH were substantial, based on our calculations. Cells recovered from patients with PPHH transcribe markers for neural progenitors, all the mature cells types of the central nervous system, and a large battery of chondroitin sulfate proteoglycan genes, including the entire aggrecan/lectican family. These cells proliferated in culture, and precursor markers were detected by Western blotting, immunocytochemistry, and cytometry. Cells could not be cultured from control patients....Continue Reading

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Citations

Aug 27, 2013·Translational Stroke Research·Jennifer StrahleGuohua Xi
Sep 9, 2011·Science Translational Medicine·Yun C YungJerold Chun
Jun 9, 2007·Cerebrospinal Fluid Research·Janet M Miller, James P McAllister
Mar 4, 2008·Cerebrospinal Fluid Research·Olga A de WitDeborah A Sival
Jun 17, 2015·Journal of Neuropathology and Experimental Neurology·María Montserrat GuerraEsteban M Rodríguez
Aug 15, 2009·Journal of Neuropathology and Experimental Neurology·Antonio Jesús JiménezJosé Manuel Pérez-Fígares
Jun 1, 2020·Stem Cells Translational Medicine·Beatriz Fernández-MuñozRosario Sanchez-Pernaute
May 19, 2017·Journal of Neuropathology and Experimental Neurology·James P McAllisterDavid D Limbrick

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