Neural Stem Cell-Conditioned Medium Ameliorated Cerebral Ischemia-Reperfusion Injury in Rats

Stem Cells International
HongNa YangTingyu Qu

Abstract

Our previous study suggested that NSC-CM (neural stem cell-conditioned medium) inhibited cell apoptosis in vitro. In addition, many studies have shown that neurotrophic factors and microparticles secreted into a conditioned medium by NSCs had neuroprotective effects. Thus, we hypothesized that NSC-CM had the capacity of protecting against cerebral I/R injury. Adult male Sprague-Dawley rats receiving middle cerebral artery occlusion surgery as an animal model of cerebral I/R injury were randomly assigned to two groups: the control group and NSC-CM-treated group. 1.5 ml NSC-CM or PBS (phosphate buffer saline) was administrated slowly by tail vein at 3 h, 24 h, and 48 h after ischemia onset. NSC-CM significantly ameliorated neurological defects and reduced cerebral infarct volume, accompanied by preserved mitochondrial ultrastructure. In addition, we also found that NSC-CM significantly inhibited cell apoptosis in the ischemic hemisphere via improving the expression of Bcl-2 (B-cell lymphoma-2). NSC-CM might be an alternative and effective therapeutic intervention for ischemic stroke.

References

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Citations

Apr 29, 2020·Biotechnology Letters·Hamid Ahmadian-MoghadamMohammad-Reza Zarrindast
Apr 9, 2020·Frontiers in Neurology·Linda OttoboniGianvito Martino
Dec 1, 2020·Frontiers in Cellular Neuroscience·Cory M WillisStefano Pluchino
May 6, 2021·International Journal of Molecular Sciences·Diana SalikhovaDmitry Goldshtein

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Methods Mentioned

BETA
protein assay
electron microscopy

Software Mentioned

GraphPad Prism
ImageJ

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