Neural stem cell delivery to the spinal cord in an ovine model of fetal surgery for spina bifida

Surgery
Dario O FauzaEvan Y Snyder

Abstract

We introduce the notion of prenatal neural stem cell (NSC) delivery to the spinal cord as an adjuvant to fetal repair of spina bifida. Fetal lambs with experimental myelomeningocele (MMC; n = 25) were divided in 3 groups: group I, no repair; group II, standard surgical MMC coverage; and group III, MMC coverage plus delivery of a murine NSCs clone into the spinal cord defect. Donor cells constitutively expressed lacZ encoding for Escherichia coli beta-galactosidase, yet they were further labeled by exposure to either BrdU and/or to the fluorescent membrane dye PKH-26. Blinded initial clinical evaluations and multiple spinal cord analyses were undertaken soon after birth. Both survival and the incidence of major paraparesis were significantly worse in group I compared with groups II and III. In group III, NSC density was highest within the most damaged areas of the spinal cord, with selective engraftment within those regions. Donor NSCs retained an undifferentiated state in vivo, producing neurotrophic factors within the defect. No animals in group III had a worsened condition following this intervention. Neural stem cells retain an undifferentiated state and produce neurotrophic factors in the short term after delivery to the fe...Continue Reading

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Citations

Jun 29, 2011·Future Neurology·Payam SaadaiDiana L Farmer
Sep 11, 2012·Stem Cells and Development·Christopher G TurnerDario O Fauza
Jun 12, 2012·Clinics in Perinatology·Payam Saadai, Diana L Farmer
Dec 14, 2011·Journal of Pediatric Surgery·Payam SaadaiDiana L Farmer
Jun 30, 2009·Clinics in Perinatology·Shinjiro Hirose, Diana L Farmer
Jun 30, 2009·Clinics in Perinatology·Christopher G B Turner, Dario O Fauza
Jan 1, 2015·Nature Reviews. Disease Primers·Andrew J CoppGary M Shaw
Apr 4, 2015·Journal of Pediatric Surgery·Erin G BrownJacqueline C Bresnahan
Sep 25, 2012·BMC Veterinary Research·Jaber LyahyaiInmaculada Martín-Burriel
Jul 11, 2009·The Journal of Bone and Joint Surgery. American Volume·Donald S BaeRussell W Jennings
Apr 26, 2015·Stem Cells Translational Medicine·Aijun WangDiana L Farmer
Jul 15, 2015·Einstein·Nicole Silva Bevilacqua, Denise Araujo Lapa Pedreira
Sep 10, 2013·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Ki-Bum SimKyu-Chang Wang
Dec 12, 2018·Neural Regeneration Research·Connor LongAijun Wang
Aug 2, 2021·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Lourenço SbragiaHélio Rubens Machado

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