Neural stem cell transplantation in mouse brain

Current Protocols in Neuroscience
Jean-Pyo LeeEvan Y Snyder

Abstract

Neural stem cells (NSCs) are the most primordial, least committed cells of the nervous system, and transplantation of these multipotent cells holds the promise of regenerative therapy for many central nervous system (CNS) diseases. This unit describes methods for NSC transplantation into neonatal mouse pups, embryonic mouse brain, and adult mouse brain. A description of options for detection of labeled donor cells in engrafted mouse brain is provided along with an example protocol for detecting lacZ-expressing cells in situ. Also included is a protocol for preparing NSCs for transplantation.

References

Jan 1, 1991·Annual Review of Biochemistry·E F Neufeld
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·B D YandavaE Y Snyder
May 3, 2000·The European Journal of Neuroscience·J M AuerbachR D McKay
Sep 27, 2000·Proceedings of the National Academy of Sciences of the United States of America·R WadaR L Proia
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yang D TengEvan Y Snyder
May 23, 2002·Human Molecular Genetics·Rachel MyerowitzRichard L Proia
Dec 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jaime ImitolaSamia J Khoury
Jul 5, 2005·Experimental Neurology·Mark A ParkerEvan Y Snyder

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Citations

Jan 29, 2020·Journal of Biomedical Science·Yoshihito HayashiKuen-Jer Tsai
Sep 3, 2009·Current Protocols in Stem Cell Biology·Araceli Espinosa-JeffreyJean de Vellis
Jun 14, 2018·Stem Cell Research & Therapy·Austin C BoeseJean-Pyo Lee
Nov 1, 2016·Current Protocols in Stem Cell Biology·Araceli Espinosa-JeffreyJean de Vellis
Aug 18, 2016·Current Protocols in Stem Cell Biology·Araceli Espinosa-JeffreyJean de Vellis
Jul 25, 2021·International Journal of Molecular Sciences·Milton H Hamblin, Jean-Pyo Lee

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