Neural stem cells in the subventricular zone are resilient to hypoxia/ischemia whereas progenitors are vulnerable.

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
Michael J RomankoSteven W Levison

Abstract

Perinatal hypoxic-ischemic (H/I) brain injury remains a major cause of neurologic disability. Because we have previously demonstrated that this insult depletes cells from the subventricular zone (SVZ), the goal of the present investigation was to compare the relative vulnerability to H/I of neural stem cells versus progenitors. The dorsolateral SVZs of P6 rats were examined at 2 to 48 hours of recovery from H/I using hematoxylin and eosin, in situ end labeling (ISEL), terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate-biotin nick end labeling (TUNEL), electron microscopy, and immunofluorescence. Pyknotic nuclei and ISEL cells were observed by 4 hours of recovery, peaked at 12 hours, and persisted for at least 48 hours. Many active-caspase-3 cells were observed at 12 hours and they comprised one third of the total TUNEL population. Electron microscopy revealed that hybrid cell deaths predominated at 12 hours of recovery. Importantly, few dying cells were observed in the medial SVZ, where putative stem cells reside, and no nestin medial SVZ cells showed caspase-3 activation. By contrast, active-caspase-3/PSA-NCAM progenitors were prominent in the lateral SVZ. These data demonstrate that early progenito...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Jan 1, 1965·Zeitschrift Für Anatomie Und Entwicklungsgeschichte·J Cammermeyer
Feb 1, 1981·Annals of Neurology·J E RiceJ B Brierley
Jul 1, 1995·Journal of Neuroscience Research·J B Grinspan, B Franceschini
Jan 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J H WijsmanJ H van Dierendonck
May 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K FehselV Kolb-Bachofen
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C Lois, A Alvarez-Buylla
Jan 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S J VannucciR C Vannucci
Jan 1, 1996·Biology of the Neonate·R A SheldonD M Ferriero
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·F Doetsch, A Alvarez-Buylla
Sep 26, 1997·The Journal of Comparative Neurology·R W Guillery, K Herrup
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·F DoetschA Alvarez-Buylla
Sep 20, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·B R HuB K Siesjö
Jan 13, 2001·Brain Research. Developmental Brain Research·M Puka-SundvallH Hagberg
Feb 17, 2001·Molecular and Cellular Neurosciences·A KawaguchiH Okano
Mar 20, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R P SkoffF S Silverstein
Mar 29, 2001·Biology of the Neonate·X WangK Blomgren
Apr 20, 2001·Brain Research. Molecular Brain Research·D C HarrisonK L Philpott
Oct 13, 2001·Developmental Neuroscience·F J NorthingtonL J Martin
Mar 29, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ramanjit GillHansruedi Loetscher
Mar 18, 2003·Developmental Neuroscience·Raymond P Rothstein, Steven W Levison
May 9, 2003·Pediatric Research·Stephane V SizonenkoChris Williams
Sep 11, 2003·Alcoholism, Clinical and Experimental Research·Hsiao-Nan HaoWilliam D Lyman

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Citations

Jun 7, 2005·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yufeng ZhouZhidan Xiang
Oct 8, 2005·International Journal of Legal Medicine·Robert E Feldmann, Rainer Mattern
Oct 13, 2010·Neuroreport·Johan JäderstadEric Herlenius
Jan 25, 2011·Fiziologiia cheloveka·K A Lebedev
Jun 9, 2007·Neonatology·A Alvarez-DíazF J Alvarez-Díaz
Dec 17, 2010·Developmental Neuroscience·Matthew V CoveySteven W Levison
May 10, 2005·Pediatric Research·Yun Sil ChangDonna M Ferriero
Jul 26, 2008·Molecular Medicine·Daniele BottaiAlfredo Gorio
Oct 11, 2014·The International Journal of Biochemistry & Cell Biology·Stuart HodgettsPilar Blancafort
Jun 6, 2007·Journal of Neuropathology and Experimental Neurology·Maria SpieglerChristiane Charriaut-Marlangue
Jun 16, 2009·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Larissa Joy ChristophidisArjan Scheepens
Jun 17, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Robin J Lichtenwalner, Jack M Parent
Feb 26, 2010·The Journal of Investigative Dermatology·Nityanand MaddodiVijayasaradhi Setaluri
May 2, 2015·Brain Research·Ruihe Lin, Lorraine Iacovitti
Oct 10, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·John D E BarksFaye S Silverstein
Jun 8, 2007·Seminars in Fetal & Neonatal Medicine·Reaz VawdaHuseyin Mehmet
Apr 25, 2007·Experimental Eye Research·Guanting QiuSriniVas R Sadda
May 29, 2009·The European Journal of Neuroscience·Takayuki NakagomiTomohiro Matsuyama
Aug 23, 2007·Journal of Neurochemistry·Michael J RomankoSteven W Levison
Mar 15, 2011·The European Journal of Neuroscience·Stephanie Z YoungAngélique Bordey
Jan 28, 2010·The European Journal of Neuroscience·Daisuke NakayamaAkihiko Taguchi
Nov 16, 2004·Ageing Research Reviews·Christine Y Brazel, Mahendra S Rao
Aug 29, 2006·Neuroscience·M L V DizonF G Szele
Mar 29, 2006·Neuroscience Letters·Maryam FaizBerta González
Oct 12, 2005·Brain Research. Brain Research Reviews·Frances J NorthingtonLee J Martin
Oct 7, 2015·Frontiers in Neurology·Joanne O DavidsonAlistair J Gunn

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Methods Mentioned

BETA
electron microscopy

Software Mentioned

IP Lab
Stat View

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