Neural transmembrane protease and endothelial Gs protein activation in cell contact-dependent signaling between neural stem/progenitor cells and brain endothelial cells.

The Journal of Biological Chemistry
Hsiu-Hui Tung, Sheau-Ling Lee

Abstract

Vasculature is an important component of the neural stem cell niche in brain. It regulates neural stem/progenitor (NS/P) cell self-renewal, differentiation, and migration. In the neurogenic niches of adult brain, NS/P cells lie close to blood vessels, and proliferating NS/P cells frequently contact the vasculature. In the present study we showed that NS/P cells in co-culture with brain endothelial (bEND) cells activated endothelial G proteins and p38 mitogen-activated protein kinase (p38 MAPK) and stimulated cytokine/chemokine expression. These NS/P cell-induced endothelial responses took place during NS/P cell and bEND cell direct contact and were critically dependent on the expression of the type II transmembrane serine protease matriptase (MTP) by NS/P cells, because knocking down of MTP in NS/P cells impaired and re-expression of MTP restored their ability to induce endothelial cytokine/chemokine expression, p38 MAPK, or G protein activation. Cholera toxin blocked NS/P cell-induced endothelial responses, suggesting that the endothelial G protein activated by NS/P MTP is in the G(s) subfamily. The addition of p38 MAPK inhibitor impaired NS/P cell-induced endothelial cytokine/chemokine expression. The known G protein-coupled ...Continue Reading

References

Jul 1, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·K H SchäferS Rose-John
Sep 7, 2000·The Journal of Comparative Neurology·T D PalmerF H Gage
Nov 2, 2001·Nature·S Temple
May 31, 2002·Pharmacological Reviews·Morley D Hollenberg, Steven J Compton
Dec 18, 2002·Cytokine & Growth Factor Reviews·Moira SauanePaul B Fisher
Jan 14, 2003·Nature Biotechnology·Qi-Long YingAustin Smith
Sep 11, 2003·The Journal of Pharmacology and Experimental Therapeutics·Joann Trejo
Mar 9, 2004·Neuron·Arturo Alvarez-Buylla, Daniel A Lim
Jan 13, 2006·British Journal of Pharmacology·Graeme Milligan, Evi Kostenis
Jan 26, 2006·Experimental Cell Research·Gabriella HonethChristian Kjellman
Jun 2, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lei WangMichael Chopp
Jun 16, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Toru YamashitaKazunobu Sawamoto
Jun 1, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Serena BovettiAdam C Puche
May 13, 2008·Experimental Hematology·Cheng-Ya DongXiao-Tong Ma
Sep 13, 2008·Cell Stem Cell·Masoud TavazoieFiona Doetsch
Oct 31, 2008·Molecular Biology of the Cell·Omedul IslamKlaus Heese
Apr 14, 2009·Cancer Gene Therapy·L N KaliberovaS A Kaliberov
Sep 18, 2009·Annals of the New York Academy of Sciences·Stéphanie LacotteHélène Dumortier
Dec 15, 2010·The Journal of Biological Chemistry·Jung-Da FangSheau-Ling Lee

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Citations

Mar 25, 2014·Journal of Molecular Neuroscience : MN·Yebing ChenHuanxiang Zhang
Dec 20, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jung-Da FangSheau-Ling Lee
Aug 13, 2020·Signal Transduction and Targeted Therapy·Zhaoqing WangXiyun Yan

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