Neurally Released GABA Acts via GABAC Receptors to Modulate Ca2+ Transients Evoked by Trains of Synaptic Inputs, but Not Responses Evoked by Single Stimuli, in Myenteric Neurons of Mouse Ileum

Frontiers in Physiology
Katerina KoussoulasJaime P P Foong

Abstract

γ-Aminobutyric Acid (GABA) and its receptors, GABAA,B,C, are expressed in several locations along the gastrointestinal tract. Nevertheless, a role for GABA in enteric synaptic transmission remains elusive. In this study, we characterized the expression and function of GABA in the myenteric plexus of the mouse ileum. About 8% of all myenteric neurons were found to be GABA-immunoreactive (GABA+) including some Calretinin+ and some neuronal nitric oxide synthase (nNOS+) neurons. We used Wnt1-Cre;R26R-GCaMP3 mice, which express a genetically encoded fluorescent calcium indicator in all enteric neurons and glia. Exogenous GABA increased the intracellular calcium concentration, [Ca2+]i of some myenteric neurons including many that did not express GABA or nNOS (the majority), some GABA+, Calretinin+ or Neurofilament-M (NFM)+ but rarely nNOS+ neurons. GABA+ terminals contacted a significantly larger proportion of the cell body surface area of Calretinin+ neurons than of nNOS+ neurons. Numbers of neurons with GABA-induced [Ca2+]i transients were reduced by GABAA,B,C and nicotinic receptor blockade. Electrical stimulation of interganglionic fiber tracts was used to examine possible effects of endogenous GABA release. [Ca2+]i transients e...Continue Reading

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Citations

Jan 8, 2020·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Jing DiXiaohong Li
May 12, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lin Y HungJaime P P Foong
Feb 6, 2020·Protoplasma·Gusel V Sibgatullina, Artem I Malomouzh
Mar 11, 2020·Nature Reviews. Gastroenterology & Hepatology·Nick J Spencer, Hongzhen Hu
Mar 23, 2021·Frontiers in Microbiology·Eric Banan-Mwine DaliriDeog-Hwan Oh

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

Imaris x64
Zen
GraphPad Prism
GraphPad
IGOR Pro
Imaris rendering

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