Neuregulin-1 regulates the expression of Akt, Bcl-2, and Bad signaling after focal cerebral ischemia in rats

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Wen-Ping GuoJian-Zhong Wu

Abstract

Neuregulin-1 (NRG-1) is a member of the epidermal growth factor family. Our previous study showed that NRG-1 protected neurons from apoptosis following focal cerebral ischemia by the inhibition of caspase-3 and TNF-alpha expression. However, the molecular signaling mechanisms for this action of NRG-1 following cerebral ischemia are not fully understood. Presently, activation of the PI3K/Akt pathway has been implicated as a major contributor to neuronal survival after an ischemic insult. In the present study, we investigated whether NRG-1 modulates the activation of Akt and its downstream targets Bad and Bcl-2 expression after transient focal cerebral ischemia by intraluminal blockade of the middle cerebral artery. Western blot was employed to analyze the change of phosphorylated Akt (p-Akt) expression; reverse transcription and polymerization chain reaction (RT-PCR) were used to measure changes of Bcl-2 mRNA. The level of phosphorylation of Bad (p-Bad) was determined using an enzyme-linked immunosorbent assay (ELISA). Our results showed that recombinant human NRG-1(3.0 ng.kg-1) significantly increased the expression of p-Akt protein, Bcl-2 mRNA, and the level of p-Bad, respectively, whereas administration of LY294002, a specifi...Continue Reading

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Citations

Jan 18, 2014·Journal of Neuroinflammation·Wesley SolomonJonathan K Stiles
Nov 14, 2013·Cardiovascular Drugs and Therapy·Afthab HussainHelen L Maddock
Sep 28, 2012·Cellular and Molecular Life Sciences : CMLS·Xiaozhou HeYing Xia
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Feb 10, 2016·The Journal of Physiology·Arsalan Alizadeh, Soheila Karimi-Abdolrezaee
Nov 14, 2013·Experimental and Therapeutic Medicine·Bingong LiMinghui Zeng

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