Neuregulin isoforms in dorsal root ganglion neurons: effects of the cytoplasmic domain on localization and membrane shedding of Nrg-1 type I

Journal of Neuroscience Research
Zhiyou ZhangD J Carey

Abstract

Embryonic sensory neurons express membrane-anchored growth factors that stimulate proliferation and differentiation of Schwann cells. The most important of these are members of the neuregulin-1 (Nrg-1) family that activate the erbB2/erbB3 receptor kinase on Schwann cells. Nrg-1 growth factors display a complex pattern of alternative mRNA splicing. We investigated the expression of the Nrg-1 type I in rat embryo dorsal root ganglion (DRG) neurons. Nrg-1 type I mRNA was abundantly expressed in DRG neurons; molecular cloning identified three distinct isoforms. The most prominent structural difference produced by alternative splicing was truncation of the C-terminal cytoplasmic domain. In sensory neurons and other cells, Nrg-1 type I proteins with the full-length 374-amino-acid cytoplasmic domain were expressed on the cell surface. In contrast, an isoform with a partially truncated cytoplasmic domain was retained in an intracellular compartment. Deletion studies demonstrated the presence of a cryptic intracellular retention signal that was exposed in the truncated cytoplasmic domain. Cell surface Nrg-1 type I molecules were subject to protease-dependent release of the biologically active ectodomain. As a consequence of their intrac...Continue Reading

References

Aug 21, 1975·Nature·P M Wood, R P Bunge
May 22, 1992·Science·W E HolmesG D Lewis
Nov 23, 1995·Nature·D Meyer, C Birchmeier
Apr 21, 1995·The Journal of Biological Chemistry·E G BarbacciG C Andrews
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T K MorrisseyR P Bunge
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·T C HeB Vogelstein
Oct 17, 1998·Molecular and Cellular Biology·M RahmatullahD J Carey
Mar 8, 2000·The Journal of Cell Biology·A N GarrattC Birchmeier
Mar 22, 2003·Experimental Cell Research·Douglas L Falls
Jun 25, 2003·The Journal of Cell Biology·Jianxin BaoDavid A Talmage
Mar 27, 2004·Science·Galin V MichailovKlaus-Armin Nave
May 5, 2004·The Journal of Biological Chemistry·Indranil DasJan Kitajewski
Jun 24, 2004·Neurobiology of Aging·Woei-Cherng ShyuHung Li
Sep 24, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Richard J Pearson, Steven L Carroll
Sep 1, 2005·Neuron·Carla TaveggiaJames L Salzer

❮ Previous
Next ❯

Citations

Feb 5, 2008·Schizophrenia Research·Victor Z ChongCynthia Shannon Weickert
Feb 18, 2009·The FEBS Journal·Christian FreeseKristina Endres
Jul 13, 2011·The Prostate·Gaëlle FromontJacques Irani
Oct 29, 2014·Molecular Psychiatry·D-M YinC Bergson
Feb 25, 2017·Glia·Jennifer Soto, Paula V Monje
Jun 18, 2020·Translational Psychiatry·Liang Shi, Clare M Bergson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.