Neurite outgrowth is enhanced by anti-idiotypic monoclonal antibodies to the ganglioside GM1

Experimental Neurology
M J Riggott, W D Matthew

Abstract

Exogenously added gangliosides enhance sprouting, neurite outgrowth, and other neuronal activities; this effect may be initiated when a ganglioside binds to a membrane protein or when a ganglioside intercalates into the plasma membrane. To test whether binding to membrane proteins is sufficient for ganglioside-mediated activity, anti-idiotypic antibodies were generated that mimic the functional binding sites of the ganglioside GM1 as described by M. J. Riggott and W. D. Matthew (1996, Glycobiology, 6, 581-589). These anti-idiotypic antibodies are proteinaceous probes that model the biochemical and biological effects of gangliosides. Those anti-idiotypic ganglioside (AIG) monoclonal antibodies (mAb's) were selected based on their ability to bind a known GM1 binding protein, the beta-subunit of cholera toxin. These studies described neuronal cell surface proteins that were identified by immunocytochemistry and Western blotting using these AIG mAb's. Here we show that AIG mAb's mimic the functional properties of GM1 in that they facilitate neurite outgrowth from central and peripheral nervous system neurons in in vitro bioassays. In addition, AIG mAb binding modulates second messenger activity, suggesting that membrane protein bin...Continue Reading

References

May 13, 1977·Brain Research·G A Banker, W M Cowan
Sep 1, 1992·Neuron·J Schlessinger, A Ullrich
Mar 16, 1992·Neuroscience Letters·S A LiptonC J Barnstable
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L J KornbergR L Juliano
Apr 20, 1990·Cell·A Ullrich, J Schlessinger
Jan 1, 1989·Methods in Enzymology·Y IgarashiS Hakomori
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A C CuelloD Maysinger
Dec 15, 1986·Chemistry and Physics of Lipids·P H Fishman
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P WalickeR Guillemin
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T C ChanhR C Kennedy
Jul 1, 1987·Journal of Cellular Biochemistry·R Briones-UrbinaN R Farid
Jun 26, 1987·Journal of Immunological Methods·W D Matthew, A W Sandrock
May 1, 1986·Scientific American·S Hakomori
Jan 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·B Rotman, B W Papermaster
Nov 1, 1984·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·N R FaridM N Islam
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·W D Matthew, P H Patterson
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M Schwartz, N Spirman
Oct 30, 1981·Science·F J RoisenG Yorke
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·E B DreyerS A Lipton
Nov 1, 1993·The Journal of Cell Biology·D Y Wu, D J Goldberg
Sep 1, 1994·Trends in Biotechnology·W C DougallM I Greene
Feb 17, 1995·The Journal of Biological Chemistry·G FerrariL A Greene

❮ Previous
Next ❯

Citations

Jul 21, 1998·Annals of the New York Academy of Sciences·R W LedeenY Fang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochemical and Biophysical Research Communications
E Albi, M P Magni
International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
M S CannellaR W Ledeen
© 2021 Meta ULC. All rights reserved