Neurite outgrowth of neural progenitors in presence of inhibitory proteoglycans

Journal of Neurotrauma
Jill SeeItzhak Fischer

Abstract

Attempts to promote host regeneration after spinal cord injury (SCI) have often resulted in poor axon extension due to formation of a glial scar, which creates a dense physical barrier around the injury and contains molecules that inhibit regeneration and repair of adult injured axons. Previous studies have shown that, while transplants of multipotent neural stem cells (NSC) integrate poorly in the injury site, the use of neuronal-restricted precursor cells (NRP) together with glial-restricted precursor cells (GRP) allow differentiation and integration of neurons, possibly because NRP are able to overcome chondroitin sulfate proteoglycan (CSPG) inhibition. To investigate this possibility, we grew mixed cultures of NRP/GRP on CSPG at inhibitory concentrations, using embryonic hippocampal cultures as controls. We found that NRP/GRP grown on CSPG survive and differentiate into neurons with no significant changes in neurite length, relative to growth on control polylysine substrate, and in contrast to a significant inhibition of axon growth in hippocampal cultures grown on CSPG-coated substrate. There was, however, a significant decrease in neurite number and branching in both cultures, indicating that CSPG also has important effec...Continue Reading

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G S EisenbarthM Nirenberg
Jun 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·D M SnowP C Letourneau
Sep 9, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M DomowiczN B Schwartz
Apr 12, 2002·Nature·Elizabeth J BradburyStephen B McMahon
Apr 29, 2006·Journal of Biology·Jeannette E DaviesStephen J A Davies
Jun 15, 2006·Journal of Cell Science·Feng-Quan ZhouWilliam D Snider
Jan 20, 2007·Biochemical and Biophysical Research Communications·Karina T WrightWilliam E B Johnson
Mar 31, 2007·Journal of Neurochemistry·Gabrielle M CuringaGeorge M Smith
Apr 9, 2008·Annals of Biomedical Engineering·Grace N LiDiane Hoffman-Kim
Aug 18, 2009·International Review of Neurobiology·Damien P Kuffler

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Citations

Sep 8, 2012·Molecular Neurobiology·Pham Ngoc Chien, Seong Eon Ryu
Mar 25, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joseph F BonnerItzhak Fischer
Sep 17, 2011·Neuroscience Research·Eri Fusaoka-NishiokaToshio Imai
Apr 23, 2015·Experimental Neurology·Scott M Dyck, Soheila Karimi-Abdolrezaee
Mar 12, 2015·Journal of Molecular Neuroscience : MN·Chao-Jin XuWei-Lin Jin
Jun 3, 2021·Cell and Tissue Research·Amanda Phuong TranJerry Silver

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