PMID: 9552165Apr 29, 1998Paper

Neurite outgrowth, RGD-dependent, and RGD-independent adhesion of identified molluscan motoneurons on selected substrates

Journal of Neurobiology
W C WilderingA G Bulloch

Abstract

The extracellular matrix (ECM) provides structural support to cells and tissues and is involved in the regulation of various essential physiological processes, including neurite outgrowth. Most of the adhesive interactions between cells and ECM proteins are mediated by integrins. Integrins typically recognize short linear amino acid sequences in ECM proteins, one of the most common being Arginine-Glycine-Aspartate (RGD). The present study investigated neurite outgrowth and adhesion of identified molluscan neurons on a selection of substrates in vitro. Involvement of RGD binding sites in adhesion to the different substrates was investigated using soluble synthetic RGD peptides. The cells adhered to native (i.e., nondenatured) laminin and type IV collagen, but not to native plasma fibronectin. Denaturation of fibronectin dramatically enhanced cell adhesion. Only the adhesion to denatured fibronectin was inhibited by RGD peptides, indicating that denaturation uncovers a RGD binding site in the protein. Laminin as well as denatured fibronectin, but not type IV collagen, induced neurite outgrowth from a percentage of the RPA neurons. These results demonstrate that molluscan neurons can attach to various substrates using both RGD-dep...Continue Reading

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Oct 16, 2007·Annals of Biomedical Engineering·Matthew J Blewitt, Rebecca Kuntz Willits
Aug 28, 2003·Biosensors & Bioelectronics·Abu Bakar Md IsmailHiroo Iwata
Jan 20, 2007·Biopolymers·Mayumi MochizukiMotoyoshi Nomizu
Mar 2, 2016·Biomedical Materials·Jun Hyuk BaeBong Geun Chung
Mar 14, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Afroza SharminEiry Kobatake

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