PMID: 9183691Jun 2, 1997Paper

Neuritic differentiation and synaptogenesis in serum-free neuronal cultures of the rat cerebral cortex

The Journal of Comparative Neurology
Ana D De LimaThomas Voigt

Abstract

To better understand the dynamics of the cellular processes involved in early neocortical development, we studied the neuritic differentiation and synaptogenesis of dispersed neurons grown in serum-free cultures under a wide variety of culture conditions. Microtubule-associated protein (MAP2), phosphorylated neurofilament (SMI 31) and synaptophysin immunocytochemistry was complemented with time-lapse studies. During the first week in vitro dissociated cortical neurons developed from roundish cells without processes to neurons with axons and differentiated dendrites, going through five distinct phases. The sequence of these phases was unaltered in a wide range of culturing methods, but the timing of the steps varied among cultures started with different cell densities. Synaptic terminals were first observed after 3-4 days in vitro, coincident with the beginning of dendritic differentiation. Synaptogenesis progressed at least until the end of the third week in vitro, despite a decline in cell density during the second week in vitro. The process of cellular differentiation of cerebral cortical neurons in vitro resembled the development of these cells in the intact tissue, suggesting that organized cell migration is not a prerequis...Continue Reading

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J E Bottenstein, G H Sato
Jun 15, 1978·The Journal of Comparative Neurology·G M Shoukimas, J W Hinds
Aug 21, 1992·Brain Research. Developmental Brain Research·C C Stichel, H W Müller
Jan 1, 1992·Annual Review of Neuroscience·L C Katz, E M Callaway
Sep 1, 1990·The Journal of Comparative Neurology·C Darian-SmithS S Cheema
Dec 17, 1991·Brain Research. Developmental Brain Research·C C Stichel, H W Müller
Dec 8, 1991·The Journal of Comparative Neurology·T Voigt, A D de Lima
Sep 15, 1990·Experientia·C Walsh, C L Cepko
Sep 1, 1990·The Journal of Comparative Neurology·M Marín-Padilla
Jan 1, 1989·Developmental Neuroscience·D C Turner, L A Flier
Jan 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K H Jones, J A Senft
Jan 1, 1989·Developmental Neuroscience·G C SephelH K Kleinman
Oct 1, 1986·Journal of Neurochemistry·P KnausH Rehm
Jan 1, 1969·Zeitschrift Für Anatomie Und Entwicklungsgeschichte·D K Morest
Jun 1, 1980·The Journal of Cell Biology·K D McCarthy, J de Vellis
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H W MüllerW Seifert
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L A Sternberger, N H Sternberger
Nov 1, 1994·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·G J RamakersG J Boer
Jan 1, 1994·The European Journal of Neuroscience·M Götz, J Bolz

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Citations

Mar 26, 2011·EURASIP Journal on Bioinformatics & Systems Biology·J AćimovićM-L Linne
Jan 29, 2013·The Journal of Biological Chemistry·Shekhar SahaSiddhartha S Jana
Sep 12, 2002·Experimental Animals·Takayuki NegishiYasuhiro Yoshikawa
Mar 1, 2012·Neurochemistry International·Takahiro TsujiHaruhiro Higashida
Mar 1, 2011·Toxicology and Applied Pharmacology·Joshua A HarrillWilliam R Mundy
Oct 26, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Joshua A HarrillWilliam R Mundy
Aug 28, 2014·The Journal of Comparative Neurology·Lauren G FriedmanDeanna L Benson
Jul 3, 2002·Molecular and Cellular Neurosciences·Nobuyuki FukushimaJerold Chun
Feb 14, 2015·In Vitro Cellular & Developmental Biology. Animal·Joshua A HarrillWilliam R Mundy
Feb 17, 2015·Molecular and Cellular Neurosciences·Mariana BrizuelaTracey C Dickson
Oct 14, 2009·Neurobiology of Disease·Vien Nguyen, Patrick S McQuillen
Mar 1, 2012·Current Biology : CB·Witchuda SaengsawangErik W Dent
Nov 9, 2007·Neuron·Adam V KwiatkowskiFrank B Gertler
Mar 14, 2017·Scientific Reports·Tania M FowkeJustin M Dean
Jan 25, 2007·Cell Motility and the Cytoskeleton·Matilda A HaasTracey C Dickson
Jan 24, 2007·The European Journal of Neuroscience·Ana D de LimaThomas Voigt
Nov 21, 2007·Nature Cell Biology·Erik W DentFrank B Gertler
Dec 17, 2008·Developmental Neurobiology·Ana D de LimaThomas Voigt
May 1, 1997·The European Journal of Neuroscience·T VoigtA Dolabela de Lima
Sep 9, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·D M KerkovichJ Busciglio
Jan 16, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Vincent LaudenbachPierre Gressens
Nov 17, 2020·Biomedical Engineering Letters·Varadraj N Vernekar, Michelle C LaPlaca

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