Neuro- and hepatic toxicological profile of (S)-2,4-diaminobutanoic acid in embryonic, adolescent and adult zebrafish

Journal of Applied Toxicology : JAT
Rosa-Maria FerraiuoloJohn F Trant

Abstract

(S)-2,4-Diaminobutanoic acid (DABA) is a noncanonical amino acid often co-produced by cyanobacteria along with β-N-methylamino-l-alanine (BMAA) in algal blooms. Although BMAA is a well-established neurotoxin, the toxicity of DABA remains unclear. As part of our development of biocompatible materials, we wish to make use of DABA as both a building block and as the end-product of enzymatically induced depolymerization; however, if it is toxic at very low concentrations, this would not be possible. We examined the toxicity of DABA using both in vivo embryonic and adult zebrafish models. At higher sublethal concentrations (700 μm), the fish demonstrated early signs of cardiotoxicity. Adolescent zebrafish were able to tolerate a higher concentration. Post-mortem histological analysis of juvenile zebrafish showed no liver or brain abnormalities associated with hepato- or neurotoxicity. Combined, these results show that DABA exhibits no overt toxicity at concentrations (100-300 μm) within an order of magnitude of those envisioned for its application. This study further highlights the low cost and ease of using zebrafish as an early-stage toxicological screening tool.

References

Oct 1, 1972·Toxicology and Applied Pharmacology·C H ChenR E Koeppe
Feb 1, 1968·The Biochemical Journal·R M O'NealR E Koeppe
Sep 21, 2002·Microscopy Research and Technique·James A Lister
Nov 6, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Adrian HillAndrew Cossins
Feb 11, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Adrian J HillRichard E Peterson
Apr 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Paul Alan CoxBirgitta Bergman
Jun 15, 2006·Journal of Neuro-oncology·A Tommy BergenheimGunnar Ronquist
Aug 26, 2006·Birth Defects Research. Part A, Clinical and Molecular Teratology·Christopher TonCatherine Willett
Feb 3, 2007·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·Deok-Ho KimSang Ho Lee
Jul 1, 1977·Neuroscience Letters·P M Beart, K Bilal
Nov 12, 2013·Trends in Biotechnology·Gavin T NobleBasar Bilgicer
Nov 26, 2013·Advanced Drug Delivery Reviews·Nicolas BertrandOmid C Farokhzad
Nov 26, 2015·Journal of Toxicology·Maitham Ahmed Al-SammakKyle D Hoagland
Jan 23, 2016·Proceedings. Biological Sciences·Paul Alan CoxSandra Anne Banack

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