Neuro-muscular differentiation of adult porcine skin derived stem cell-like cells.

PloS One
Dominik LermenPaul Müller

Abstract

Due to the genetic relationship to humans, porcine stem cells are a very important model system to investigate cell differentiation, associated cell signaling pathways, and cell fate. Porcine skin derived stem cells have been isolated from mid-gestation porcine fetus recently. To our knowledge, stem cells from the skin of the adult porcine organism have not been isolated until now. Hence, to our knowledge, we here describe the isolation, expansion, characterization and differentiation of multipotent porcine skin derived stem cell-like cells (pSSCs) from the adult porcine organism for the first time. pSSCs had a spindle shaped morphology similar to mesenchymal stem cells (MSCs). They could be maintained proliferatively active in vitro for more than 120 days and were able to form colonies from single cells. pSSCs expressed Sox2 and Oct3/4, both transcription factors essential to the pluripotent and self-renewing phenotypes of embryonic stem cells, which recently gained attention due to their function in inducing pluripotent stem cells. Furthermore, the expression of the progenitor marker nestin, the somatic stem cell markers Bcrp1/ABCG2, Bmi1, and Stat3 was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in u...Continue Reading

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Citations

Nov 9, 2010·Stem Cells and Development·Paul W DyceJulang Li
Jan 9, 2014·Journal of Pharmaceutical Sciences·Dongwei ZhangChris D Rielly
Aug 7, 2010·Theriogenology·Ming-Tao Zhao, R S Prather
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Nov 20, 2019·Toxicologic Pathology·Qingqiu Yang, Mandi J Lopez

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Methods Mentioned

BETA
biopsies
biopsy
flow cytometry
FCS
PCR

Software Mentioned

Image J
CellQuest

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