Neuroanatomical and physiological evidence that the retrotrapezoid nucleus/parafacial region regulates expiration in adult rats

Respiratory Physiology & Neurobiology
Josiane N SilvaAna C Takakura

Abstract

The rostroventrolateral medulla contains two functional neuronal populations: (1) the parafacial respiratory group (pFRG) neurons and (2) the chemosensitive retrotrapezoid nucleus (RTN) neurons. Using anatomical and physiological techniques, we investigated the role of the RTN/pFRG in CO2-induced active expiration (AE) in urethane-anesthetized rats. Anterograde tracing using biotinylated dextran amine (BDA) revealed dense neuronal projections emanating from the RTN/pFRG to the caudal ventral respiratory group (cVRG), 60% of which contained vesicular glutamate transporter-2. The minority (16%) of the RTN projections to the cVRG emanated from Phox2b positive neurons. Hypercapnia (10% CO2) increased DiaEMG and elicited AbdEMG activity. Bilateral injections of muscimol (2mM) into the RTN/pFRG reduced the activation of DiaEMG (23±4%) and abolished AE-induced by chemoreflex stimulation. Taken together, these results support the presence of direct excitatory projections from RTN/pFRG neurons to cVRG expiratory premotor neurons, playing a role in the generation/modulation of AE.

References

Dec 1, 1992·Brain Research Bulletin·H OnimaruK Iwatsuki
Mar 1, 1989·The Journal of Comparative Neurology·J C SmithJ L Feldman
Sep 1, 1994·The Journal of Comparative Neurology·E G Dobbins, J L Feldman
Oct 17, 1998·Progress in Neurobiology·S Iscoe
Apr 28, 1999·Neuroscience·W Wang, G B Richerson
May 2, 2001·The Journal of Comparative Neurology·H WangP G Guyenet
Jul 23, 2002·Journal of Applied Physiology·Yasumasa OkadaFrederic L Eldridge
Jan 13, 2004·Journal of Applied Physiology·Jay B DeanRobert W Putnam
May 21, 2004·Nature Reviews. Neuroscience·George B Richerson
Nov 19, 2005·The Journal of Physiology·Wiktor A Janczewski, Jack L Feldman
Sep 8, 2006·The Journal of Comparative Neurology·Diane L RosinPatrice G Guyenet
Oct 6, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ruth L StornettaPatrice G Guyenet
Feb 13, 2007·Respiratory Physiology & Neurobiology·Makito Iizuka, Ralph F Fregosi
Mar 7, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Michal G FortunaPatrice G Guyenet
Apr 29, 2008·The Journal of Physiology·Ana C TakakuraPatrice G Guyenet
Aug 19, 2008·Respiratory Physiology & Neurobiology·George F Alheid, Donald R McCrimmon
May 8, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Stephen B G AbbottPatrice G Guyenet
Aug 21, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Matthew R HodgesGeorge B Richerson
Aug 26, 2010·The Journal of Comparative Neurology·Patrice G GuyenetDouglas A Bayliss
Sep 17, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nephtali MarinaAlexander V Gourine
Nov 12, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ana C TakakuraThiago S Moreira
Feb 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Seth D DepuyPatrice G Guyenet
Mar 19, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Silvia PagliardiniJack L Feldman
Sep 22, 2011·The Journal of Comparative Neurology·Genrieta BochorishviliPatrice G Guyenet
Nov 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Stephen B G AbbottPatrice G Guyenet
Mar 31, 2012·Brain Structure & Function·Ruth L StornettaPatrice G Guyenet
Nov 6, 2012·Annual Review of Physiology·Jack L FeldmanPaul A Gray
Dec 7, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Davi J A MoraesBenedito H Machado
Jan 16, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tyler M BastingPatrice G Guyenet
Jan 23, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Robert T R HucksteppJack L Feldman
Sep 4, 2015·Neuron·Patrice G Guyenet, Douglas A Bayliss

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Citations

Jun 15, 2016·ELife·Robert Tr HucksteppJack L Feldman
May 27, 2016·Journal of Neurophysiology·Thiago S MoreiraJose J Otero
Apr 26, 2017·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Benedito H MachadoDavi J A Moraes
May 2, 2018·Annual Review of Neuroscience·Jan-Marino Ramirez, Nathan A Baertsch
Aug 11, 2018·PloS One·Robert T R HucksteppJack L Feldman
Sep 7, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Daniel B ZoccalAna C Takakura
Feb 14, 2019·Experimental Physiology·Luiz M OliveiraAna C Takakura
Jun 27, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Josiane N SilvaAna C Takakura
Mar 17, 2017·F1000Research·Tatiana M Anderson, Jan-Marino Ramirez
Oct 27, 2017·Journal of Neurophysiology·William H BarnettDaniel B Zoccal
May 10, 2018·Nature Reviews. Neuroscience·Christopher A Del NegroJack L Feldman
Apr 9, 2020·Journal of Neurophysiology·Josiane do N SilvaAna C Takakura
Jan 28, 2021·Journal of Neurophysiology·Jaseph Soto-Perez
Mar 31, 2021·Respiratory Physiology & Neurobiology·Ana C TakakuraThiago S Moreira
Aug 28, 2021·International Journal of Molecular Sciences·Maxime LemieuxFrédéric Bretzner

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