Neurobasal medium toxicity on mature cortical neurons

Neuroreport
Daniele MaggioniArianna Scuteri

Abstract

Neurobasal medium (NBM) is a widely used medium for neuronal cultures, originally formulated to support survival of rat hippocampal neurons, but then optimized for several other neuronal subtypes. In the present study, the toxic effect of NBM on long-term cortical neuron cultures has been reported and investigated. A significant neuronal cell loss was observed 24 h after the total medium change performed at days in vitro 10. The neurotoxic effect was specifically because of NBM-A, a commercially derived modification of classic NBM, as neurons exposed to minimum essential medium for 24 h did not show the same mortality rate. We showed that the toxic effect was mediated by the N-methyl-D-aspartate receptor (NMDAr) as its inactivation partly prevented NBM-induced neuronal loss, and the addition of NMDAr activators, such as L-cysteine or glycine to minimum essential medium, reproduced the same toxicity rate observed in NBM. Besides the toxicity associated with NMDAr activation, the decreased antioxidative defenses also worsen (because of glutathione depletion) neuronal death, thus amplifying the effect of excitotoxic amino acids. Indeed, glutathione supplementation by the addition of its precursor N-acetyl-cysteine resulted in an i...Continue Reading

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Citations

Mar 15, 2016·Frontiers in Cellular Neuroscience·Marta Perez-AlcazarSebastian Illes
Aug 16, 2016·Neuroscience Letters·Susanna BacigaluppiGiovanni Tredici
Feb 28, 2020·Stem Cell Research & Therapy·Lei ZhangYunhui Liu
May 20, 2020·Frontiers in Synaptic Neuroscience·Enora MoutinVincent Compan

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