Neurobiological signatures of alcohol dependence revealed by protein profiling

PloS One
Giorgio GoriniR Dayne Mayfield

Abstract

Alcohol abuse causes dramatic neuroadaptations in the brain, which contribute to tolerance, dependence, and behavioral modifications. Previous proteomic studies in human alcoholics and animal models have identified candidate alcoholism-related proteins. However, recent evidences suggest that alcohol dependence is caused by changes in co-regulation that are invisible to single protein-based analysis. Here, we analyze global proteomics data to integrate differential expression, co-expression networks, and gene annotations to unveil key neurobiological rearrangements associated with the transition to alcohol dependence modeled by a Chronic Intermittent Ethanol (CIE), two-bottle choice (2BC) paradigm. We analyzed cerebral cortices (CTX) and midbrains (MB) from male C57BL/6J mice subjected to a CIE, 2BC paradigm, which induces heavy drinking and represents one of the best available animal models for alcohol dependence and relapse drinking. CIE induced significant changes in protein levels in dependent mice compared with their non-dependent controls. Multiple protein isoforms showed region-specific differential regulation as a result of post-translational modifications. Our integrative analysis identified modules of co-expressed prot...Continue Reading

References

Aug 1, 1997·Neuroscience·J J KrilH Cartwright
Jul 21, 1998·Annals of the New York Academy of Sciences·P R Dodd, J M Lewohl
Feb 18, 1999·Molecular and Cellular Biology·S P GygiR Aebersold
Mar 10, 2000·Biochemical and Biophysical Research Communications·V CalabreseA M Stella
Feb 21, 2002·Neuropsychology·Edith V SullivanAdolf Pfefferbaum
Jun 18, 2002·Journal of Neurochemistry·R Dayne MayfieldR Adron Harris
Jul 31, 2003·Genes, Brain, and Behavior·T J PhillipsJ C Crabbe
Sep 4, 2003·Genome Biology·Dov GreenbaumMark Gerstein
Mar 17, 2004·Neuroscience and Biobehavioral Reviews·George F KoobPietro Paolo Sanna
Mar 26, 2004·Nature Reviews. Molecular Cell Biology·Gerrit J K Praefcke, Harvey T McMahon
Oct 7, 2004·Neuropharmacology·Eric J Nestler
Nov 16, 2004·Annals of the New York Academy of Sciences·Joanne M LewohlPeter R Dodd
Nov 24, 2004·Neuroscience and Biobehavioral Reviews·Jeffrey W DalleyTrevor W Robbins
Apr 19, 2005·Psychopharmacology·Edith V Sullivan, Adolf Pfefferbaum
Oct 28, 2005·Nature Neuroscience·Eric J Nestler
Nov 18, 2005·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jianwen LiuR Dayne Mayfield
Apr 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Megan K MulliganSusan E Bergeson
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Bin Zhang, Steve Horvath
Jun 17, 2006·Annual Review of Neuroscience·Matthew J Kennedy, Michael D Ehlers
Apr 4, 2007·Alcoholism, Clinical and Experimental Research·Deborah A FinnAmanda J Roberts
Nov 23, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Leonardo PignataroNeil L Harrison
Feb 19, 2008·Psychiatry Research·Nora D VolkowGene-Jack Wang
Mar 26, 2008·British Journal of Pharmacology·R D MayfieldM A Schuckit
Dec 31, 2008·BMC Bioinformatics·Peter Langfelder, Steve Horvath
Aug 12, 2009·Pharmacology, Biochemistry, and Behavior·Richard L BellWilliam J McBride
Aug 14, 2009·Alcoholism, Clinical and Experimental Research·William C GriffinHoward C Becker
Sep 29, 2009·Trends in Neurosciences·Steven N Treistman, Gilles E Martin
Nov 20, 2009·Proteomics. Clinical Applications·Naomi EtheridgePeter R Dodd
Jan 1, 2007·Proteomics. Clinical Applications·Kimberley Alexander-KaufmanIzuru Matsumoto
Nov 1, 2007·Proteomics. Clinical Applications·Ming D Li, Ju Wang
Jan 5, 2011·Progress in Molecular Biology and Translational Science·Giorgio GoriniR Dayne Mayfield
Jan 13, 2011·Alcoholism, Clinical and Experimental Research·Megan K MulliganIgor Ponomarev
Jan 20, 2011·Nature Reviews. Genetics·Eric Huntzinger, Elisa Izaurralde
Jun 10, 2011·Alcoholism, Clinical and Experimental Research·Joanne M LewohlR Dayne Mayfield
Dec 7, 2011·Methods in Molecular Biology·Barbara D'haeneJo Vandesompele
Jan 12, 2012·Nature Reviews. Molecular Cell Biology·Shawn M Ferguson, Pietro De Camilli
Feb 4, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Igor PonomarevR Dayne Mayfield

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Citations

Nov 19, 2015·Alcoholism, Clinical and Experimental Research·Max KreifeldtCandice Contet
Dec 1, 2015·Frontiers in Cellular Neuroscience·Paolo FollesaGiorgio Gorini
Mar 4, 2017·Neuropharmacology·Anna S Warden, R Dayne Mayfield
Aug 1, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Giorgio GoriniR Dayne Mayfield
Aug 23, 2020·Brain Sciences·Lauren C Smith, Adam Kimbrough
Jan 16, 2020·Proceedings of the National Academy of Sciences of the United States of America·Adam KimbroughOlivier George
Jul 26, 2018·Neurochemical Research·Mohammed A KashemVladimir J Balcar

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Datasets Mentioned

BETA
PXD000349

Methods Mentioned

BETA
electrophoresis
protein assay
PCR
acetylation

Software Mentioned

DAVID
[UNK]
Image Quant
Bioconductor
GPS Explorer
DeCyder
qbasePLUS
MASCOT
WGCNA
SoftPower

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