PMID: 9653184Jul 8, 1998Paper

Neuroblast pattern formation: regulatory DNA that confers the vnd/NK-2 homeobox gene pattern on a reporter gene in transgenic lines of Drosophila

Proceedings of the National Academy of Sciences of the United States of America
H H SaundersMarshall Nirenberg

Abstract

DNA fragments -0.57, -2.2, -2.9, -5.3, and -8.4 kb in length from the upstream regulatory region of the vnd/NK-2 gene were cloned in the 5'-flanking region of a beta-galactosidase (beta-gal) reporter gene in the P-element pCaSpeR-AUG-beta-gal, and the effects of the DNA on the pattern and time of expression of beta-gal were determined in transgenic embryos. Embryos from 11 lines transformed with -8.4 kb of vnd/NK-2 regulatory DNA expressed beta-gal patterns that closely resemble those of vnd/NK-2. In embryos from four lines transformed with -5.3 kb of vnd/NK-2 DNA, beta-gal was found in the normal vnd/NK-2 pattern in the nerve cord but not in part of the cephalic region. beta-Gal patterns in embryos from transgenic lines containing -0.57, -2.2, or -2.9 kb of vnd/NK-2 DNA did not resemble vnd/NK-2. Null vnd/NK-2 mutant embryos containing the homozygous P-element p[-8.4 to +0.34 beta-gal] expressed little beta-gal in contrast to siblings with a wild-type vnd/NK-2 gene. We conclude that (i) the 8.4-kb DNA fragment from the vnd/NK-2 gene contains the nucleotide sequences required to generate the normal pattern of vnd/NK-2 gene expression, sequences that may be involved in the switch between neuroblast vs. epidermoblast pathways of ...Continue Reading

References

Jan 1, 1991·Annual Review of Cell Biology·S Artavanis-TsakonasR G Fehon
Jun 1, 1989·Genes & Development·N H PatelR Holmgren
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·Y Kim, M Nirenberg
Nov 4, 1988·Cell·M A Kuziora, W McGinnis
Aug 1, 1984·Cell·R E Karess, G M Rubin
Oct 22, 1982·Science·G M Rubin, A C Spradling
Jun 30, 1995·Annals of the New York Academy of Sciences·M NirenbergR Lad
Sep 1, 1984·Wilhelm Roux's Archives of Developmental Biology·Volker Hartenstein, Jose A Campos-Ortega

❮ Previous
Next ❯

Citations

Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·Xiaoping ShaoMarshall Nirenberg
May 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Lan-Hsiang WangMarshall Nirenberg
Mar 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Keita KoizumiJames A Ferretti
Sep 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Alexander Stepchenko, Marshall Nirenberg
Oct 2, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tonia Von OhlenWill Poulson
Oct 30, 2009·Journal of Neurogenetics·Doo Na KangSang-Hak Jeon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Xiaoping ShaoMarshall Nirenberg
Proceedings of the National Academy of Sciences of the United States of America
Lan-Hsiang WangMarshall Nirenberg
© 2022 Meta ULC. All rights reserved