PMID: 2107277Apr 1, 1990Paper

Neuroblastoma differentiation involves the expression of two isoforms of the alpha-subunit of Go

Journal of Neurochemistry
P BrabetV Homburger

Abstract

The regulation of GTP-binding proteins (G proteins) was examined during the course of differentiation of neuroblastoma N1E-115 cells. N1E-115 cell membranes possess three Bordetella pertussis toxin (PTX) substrates assigned to alpha-subunits (G alpha) of Go (a G protein of unknown function) and "Gi (a G protein inhibitory to adenylate cyclase)-like" proteins and one substrate of Vibrio cholerae toxin corresponding to an alpha-subunit of Gs (a G protein stimulatory to adenylate cyclase). In undifferentiated cells, only one form of Go alpha was found, having a pI of 5.8 Go alpha content increased by approximately twofold from the undifferentiated state to 96 h of cell differentiation. This is mainly due to the appearance of another Go alpha form having a pI of 5.55. Both Go alpha isoforms have similar sizes on sodium dodecyl sulfate-polyacrylamide gels, are recognized by polyclonal antibodies to bovine brain Go alpha, are ADP-ribosylated by PTX, and are covalently myristylated in whole N1E-115 cells. In addition, immunofluorescent staining of N1E-115 cells with Go alpha antibodies revealed that association of Go alpha with the plasma membrane appears to coincide with the expression of the most acidic isoform and morphological cel...Continue Reading

References

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Citations

Jun 1, 1993·Journal of Neuroscience Research·M Zubiaur, E J Neer
Dec 15, 1994·European Journal of Pharmacology·R ZumbihlB Rouot
Jun 1, 1991·Current Opinion in Neurobiology·J Bockaert
Feb 13, 2009·Neuro-Signals·Meisheng Jiang, Neil S Bajpayee
Aug 1, 1997·The American Journal of Physiology·J S Coggan, S H Thompson

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