PMID: 9646033Jul 1, 1998Paper

Neuroendocrine differentiation in Ewing's sarcomas and primitive neuroectodermal tumors revealed by reverse transcriptase-polymerase chain reaction of chromogranin mRNA

Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B
A PaganiG Bussolati

Abstract

Ewing's sarcomas (ESs), primitive neuroectodermal tumors (PNETs), and neuroblastomas (NBs) are closely related neoplasms supposedly derived from the neural crest and belonging to the family of the small blue round cell tumors of infancy and childhood. We investigated the expression of the neuroendocrine and neuroectodermal markers chromogranin A (CgA) and secretogranin II (SgII) in ESs, PNETs, and NBs, both in primitive tumors (five, nine, and four cases, respectively) and in established cell lines (three ES and two PNET cell lines). Different technical approaches, namely immunohistochemistry, Northern blot analysis, and reverse transcriptase-polymerase chain reaction (RT-PCR) were used in parallel. Chromogranin A and secretogranin II production was constantly detectable in NBs by all procedures. CgA mRNA was detectable in most ESs and PNETs only by RT-PCR, whereas SgII mRNA was detectable in some ESs and PNETs by Northern blot analysis and in all tumors by RT-PCR. CgA and SgII proteins were never detectable by immunohistochemistry in ESs and PNETs. We conclude that neuroendocrine differentiation is shared by all three tumor entities, being more overt in NBs and rudimentary in ESs and PNETs; traces of chromogranin mRNA are dete...Continue Reading

References

Mar 1, 1992·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·A PaganiG Bussolati
Sep 1, 1992·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·A PaganiG Bussolati
Aug 1, 1992·Neuroscience·H Winkler, R Fischer-Colbrie
May 10, 1991·International Journal of Cancer. Journal International Du Cancer·T SugimotoT Sawada
Jan 1, 1990·Virchows Archiv. A, Pathological Anatomy and Histopathology·S NavarroA Llombart-Bosch
Mar 1, 1990·Cancer Genetics and Cytogenetics·R NogueraT J Triche
Jan 1, 1989·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·B Wiedenmann, W B Huttner
May 18, 1989·Nature·S Kwok, R Higuchi
Dec 1, 1984·The American Journal of Surgical Pathology·R JaffeM Goodman
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Jun 1, 1995·Human Pathology·R S NelsonF B Askin
Jan 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P TerrierA Llombart-Bosch
Apr 1, 1994·Japanese Journal of Cancer Research : Gann·K KodamaY Mori
Sep 1, 1993·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·M LadanyiS C Jhanwar
Jan 1, 1993·The American Journal of Surgical Pathology·L P Dehner
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·P H SorensenT J Triche
Apr 1, 1996·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A ZoubekH Kovar

❮ Previous
Next ❯

Citations

May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert TibshiraniGilbert Chu
Oct 22, 2013·International Journal of Surgical Pathology·Helena BarrocaManuel Sobrinho-Simões
Jul 10, 2008·International Journal of Surgical Pathology·M Adib HoureihS Sankar Banerjee
Mar 14, 2018·The American Journal of Dermatopathology·Isidro MachadoAntonio Llombart-Bosch
May 25, 2010·The American Journal of Surgical Pathology·Marta BarisellaSilvana Pilotti
Apr 13, 2019·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Hannah WangGregory W Charville
Nov 9, 2012·Histopathology·W Glenn McCluggage
Feb 20, 2018·Oncology Letters·Parthik Patel, Karina Galoian
Nov 28, 2013·International Journal of Surgical Pathology·Catarina EloyManuel Sobrinho-Simões
Jul 17, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·F TimeusG Basso
Aug 6, 2021·Virchows Archiv : an International Journal of Pathology·Atsuko KasajimaGünter Klöppel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B
A PaganiG Bussolati
Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B
S NavarroA Llombart-Bosch
Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology
D C WestJ Sklar
© 2022 Meta ULC. All rights reserved