Neurofilament heavy chain expression reveals a unique parasagittal stripe topography in the mouse cerebellum.

The Cerebellum
Adrien DemillyRoy V Sillitoe

Abstract

Despite the general uniformity in cellular composition of the adult cerebellum (Cb), the expression of proteins such as ZebrinII/AldolaseC and the small heat shock protein HSP25 reveal striking patterns of parasagittal Purkinje cell (PC) stripes. Based on differences in the stripe configuration within subsets of lobules, the Cb can be further divided into four anterior-posterior transverse zones: anterior zone (AZ) = lobules I-V, central zone (CZ) = lobules VI-VII, posterior zone (PZ) = lobules VIII and anterior IX, and the nodular zone (NZ) = lobules posterior IX-X. Here we used whole-mount and tissue section immunohistochemistry to show that neurofilament heavy chain (NFH) expression alone divides all lobules of the mouse Cb into a complex series of parasagittal stripes of PCs. We revealed that the striped pattern of NFH in the vermis of the AZ and PZ was complementary to ZebrinII and phospholipase C ß3 (PLCß3), and corresponded to phospholipase C ß4 (PLCß4). In the CZ and NZ the stripe pattern of NFH was complementary to HSP25 and corresponded to PLCß3. The boundaries of the NFH stripes were not always sharply delineated. Instead, a gradual decrease in NFH expression was observed toward the edges of particular stripes, resul...Continue Reading

References

Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·N LeclercM Yamamoto
Oct 1, 1985·Journal of the Neurological Sciences·A V PlioplysR Hawkes
Jan 1, 1996·Annual Review of Neuroscience·M K Lee, D W Cleveland
Sep 15, 1998·Trends in Neurosciences·J Voogd, M Glickstein
Sep 15, 1998·Trends in Neurosciences·D Goldowitz, K Hamre
Oct 8, 1998·The Journal of Cell Biology·G A ElderR A Lazzarini
Jan 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Roy V Sillitoe, Richard Hawkes
Jun 6, 2003·Brain Research·Roy V SillitoeRichard Hawkes
Jun 3, 2004·Journal of Neurocytology·Hassan MarzbanRichard Hawkes
Oct 8, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Izumi Sugihara, Yoshikazu Shinoda
Jan 22, 2005·Progress in Brain Research·Roy V SillitoeRichard Hawkes
Sep 2, 2005·Nature Neuroscience·Jacques I Wadiche, Craig E Jahr
Mar 28, 2006·The Journal of Comparative Neurology·Justyna R SarnaRichard Hawkes
Jun 2, 2006·The Journal of Comparative Neurology·Martijn SchonewilleChris I De Zeeuw
Dec 8, 2006·Nature·Ed S LeinAllan R Jones
Dec 22, 2006·The Journal of Comparative Neurology·Izumi Sugihara, Pham Nguyen Quy
Apr 17, 2007·The Journal of Comparative Neurology·Hassan MarzbanRichard Hawkes
May 18, 2007·Annual Review of Cell and Developmental Biology·Roy V Sillitoe, Alexandra L Joyner
May 19, 2007·Brain, Behavior and Evolution·K W S AshwellC R R Watson
Apr 17, 2008·The European Journal of Neuroscience·Thomas B Shea, Walter K-H Chan

❮ Previous
Next ❯

Citations

Aug 9, 2011·The Cerebellum·John Oberdick, Roy V Sillitoe
Apr 18, 2012·Journal of Visualized Experiments : JoVE·Joshua J WhiteRoy V Sillitoe
Nov 11, 2019·Disease Models & Mechanisms·Trace L StayRoy V Sillitoe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.