Neurofilament phosphorylation in neuronal perikarya following axotomy: a study of rat spinal cord with ventral and dorsal root transection

The Journal of Comparative Neurology
H MansourD Dahl

Abstract

Rat spinal cord was stained by indirect immunofluorescence with 11 neurofilament monoclonal antibodies that recognize phosphorylated epitopes. All monoclonals were axon-specific in this location. The large motoneurons containing bundles of neurofilaments did not stain and the pattern remained unchanged after transection of the sciatic nerve in the thigh. With nine monoclonals, stained motoneurons were observed in the ventral horns 3 days, 5 days, 1 week, and 2 weeks after transection of the ventral roots close to the spinal cord. The abnormal motoneurons were typically scattered among normal (i.e., nonstained) cells. Even in animals showing the most severe reaction, the whole motoneuron population at the site of rhizotomy was not affected, stained and nonstained perikarya often coexisting side by side. Stained motoneurons were no longer observed 3 weeks after ventral root transection. Changes in neuronal immunoreactivity were also observed after dorsal root transection. However, a different population was affected, i.e., middle-sized neurons in dorsal horns and at the base of ventral horns. With two monoclonals (A9 and D21), cell bodies remained negative following all operations. It is concluded that axotomy in proximity of the...Continue Reading

References

Dec 15, 1977·The Journal of Comparative Neurology·D Dahl, A Bignami
Nov 1, 1986·Journal of Neurology, Neurosurgery, and Psychiatry·M C HaughB H Anderton
Jan 1, 1986·Experimental Cell Research·D Dahl, A Bignami
Jan 1, 1986·Journal of Neuroscience Research·D DahlA Bignami
Jan 1, 1987·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·A Bignami, D Dahl
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N H SternbergerJ Ulrich
Jan 1, 1988·Journal of Neuropathology and Experimental Neurology·D G MunozD J Selkoe
Jan 1, 1986·Journal of Neuropathology and Experimental Neurology·L C CorkD L Price
May 1, 1987·Journal of Neuropathology and Experimental Neurology·J RosenfeldD L Price
Sep 25, 1981·Science·P GambettiS C Papasozomenos
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L A Sternberger, N H Sternberger
Feb 1, 1981·The Journal of Comparative Neurology·D DahlN H Chi

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Citations

Jan 1, 1991·Acta Neuropathologica·S MurayamaK Suzuki
Aug 14, 1999·Brain Research. Developmental Brain Research·D A HouwelingE A Joosten
Jul 11, 2007·Journal of Neuropathology and Experimental Neurology·Francoise GrayHugues Chabriat
Jun 14, 2015·Experimental Eye Research·Andrea C NuschkeBalwantray C Chauhan
Mar 18, 2010·The Journal of Comparative Neurology·Michel K HerdeMarco B Rust
Apr 1, 2009·Journal of Neurotrauma·Clara PenasXavier Navarro

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