PMID: 701353Sep 1, 1978Paper

Neurofilament proteins of rat peripheral nerve and spinal cord

The Journal of Cell Biology
W W Schlaepfer, L A Freeman

Abstract

Intact neurofilaments were isolated in parallel from rat peripheral nerve and spinal cord by osmotic shock into hypotonic media containing divalent cation chelators. Isolated neurofilaments were washed and separated by multiple centrifugations in 0.1 M NaCl. Abundant intact neurofilaments were identified in the washed pellets by negative staining techniques. Their origin from neurofilaments was confirmed by immune electron microscopy. Washed neurofilaments were extracted from lipid and membranous components with 8 M urea. Analyses of neurofilament isolates on sodium dodecyl sulfate gels showed that proteins of 200,000, 150,000, and 69,000 mol wt were the major components of intact neurofilaments derived from rat peripheral and central nervous systems. These same proteins were identified in whole tissue homogenates of both sources and became enriched during the isolation of intact neurofilaments. A minor component of 64,000 mol wt arose during isolation. Other proteins were identified as contaminants. Small amounts of proteins with electrophoretic migration of tubulin and actin remain in neurofilament isolates.

References

Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S H YenM L Shelanski
Dec 15, 1977·The Journal of Comparative Neurology·D Dahl, A Bignami
Oct 15, 1977·Experimental Cell Research·P F DavisonP Cooke
Aug 1, 1978·The Journal of Cell Biology·W W Schlaepfer, S Micko
Sep 1, 1977·Journal of Neurochemistry·K IqbalR D Terry
Jul 19, 1976·Biochimica Et Biophysica Acta·G H De VriesM G Hadfield
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M L ShelanskiC R Cantor
Mar 24, 1972·Science·G H DeVriesC S Raine
Nov 1, 1973·The Journal of Cell Biology·W W Schlaepfer, R P Bunge
Jan 1, 1974·Journal of Neurobiology·P F Davison, B Winslow
Dec 17, 1971·Science·M L ShelanskiW T Norton

❮ Previous
Next ❯

Citations

Jan 1, 1987·Cell and Tissue Research·K Meller
Jan 1, 1984·Virchows Archiv. A, Pathological Anatomy and Histopathology·F CarleiV Speranza
Aug 1, 1995·Cellular and Molecular Neurobiology·H S Yin
Aug 1, 1981·Developmental Biology·S J TapscottH Holtzer
May 1, 1986·Journal of the Neurological Sciences·N L BanikK P Smith
Jan 1, 1982·Neuroscience·S TsukitaH Ishikawa
Aug 1, 1992·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·V FloresJ P Saavedra
Jan 1, 1992·Electron Microscopy Reviews·K Meller
Aug 1, 1994·Critical Reviews in Oncology/hematology·W M Molenaar, J Q Trojanowski
Dec 5, 1998·Brain Research. Developmental Brain Research·G TrainaP Bagnoli
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C WangE Lazarides
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R V ZackroffR D Goldman
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M E GoldsteinN H Sternberger
Feb 1, 1989·The Journal of Cell Biology·P J Hollenbeck
May 1, 1980·The Journal of Cell Biology·D J Fink, H Gainer
Jun 1, 1980·The Journal of Cell Biology·H CzosnekH M Wisniewski
Aug 1, 1980·The Journal of Cell Biology·M M Black, R J Lasek

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Biology of Lipids

Lipids are essential in many biological functions, including membrane structure, energy storage, and cell signaling. Find the latest research on the cell biology of lipids here.