Abstract
We previously reported a primitive cell fraction derived from human circulating CD14+ monocytes, named monocyte-derived multipotential cells (MOMC), that can differentiate along mesenchymal lineages, including bone, cartilage, fat, skeletal muscle and cardiac muscle. In this study, we investigated whether MOMC can differentiate into the neuronal lineage. MOMC were fluorescently labelled and cocultivated with a primary culture of rat neurons for up to 4 weeks. The protein and gene expressions of neuron-specific markers in the human MOMC were evaluated over time using immunohistochemistry, in situ hybridization and reverse transcription followed by PCR. Shortly after cocultivation with rat neurons, nearly all the MOMC expressed early neuroectodermal markers, Mash1, Neurogenin2 and NeuroD, together with nestin, an intermediate filament expressed in neurogenesis. After 14 days of coculture, a subpopulation of MOMC displayed a multipolar morphology with elongated neurites and expressed mature neuron-specific markers, including neurofilament, microtubule-associated protein type 2, beta3-tubulin, NeuN and Hu. Transdifferentiation of monocytes into the neuroectodermal lineage was shown by the simultaneous expression of proneural marker...Continue Reading
References
Feb 23, 1990·Cell·U LendahlR D McKay
Jun 1, 1988·Journal of Neurochemistry·J R Sanchez-RamosF Hefti
Jun 1, 1993·Experimental Neurology·C G ParamoreR D Madison
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·M A Eglitis, E Mezey
Aug 1, 2000·Experimental Neurology·J Sanchez-RamosP R Sanberg
Aug 10, 2000·Differentiation; Research in Biological Diversity·B Fernandez PujolK Havemann
Aug 10, 2000·Journal of Neuroscience Research·D WoodburyI B Black
Nov 1, 2000·Arthritis Research·N J ZvaiflerR N Maini
Dec 2, 2000·Science·T R BrazeltonH M Blau
Dec 21, 2000·JAMA : the Journal of the American Medical Association
Feb 13, 2001·Cardiovascular Research·A SchmeisserW G Daniel
Feb 24, 2001·Developmental Biology·A D SimmonsJ E Johnson
Jan 5, 2002·Differentiation; Research in Biological Diversity·J KohyamaA Umezawa
Mar 5, 2002·Nature Medicine·Cindi M MorsheadDerek van der Kooy
Mar 6, 2002·The European Journal of Neuroscience·Sonia BonillaSalvador Martínez
Apr 5, 2002·Nature·Naohiro TeradaEdward W Scott
Apr 5, 2002·Nature·Qi-Long YingAustin G Smith
Jun 22, 2002·Nature·Yuehua JiangCatherine M Verfaillie
Aug 7, 2002·The American Journal of Pathology·Hiroaki KodamaKazuto Yamazaki
Sep 7, 2002·Science·Amy J WagersIrving L Weissman
Dec 31, 2002·Experimental Neurology·Peter KabosJohn S Yu
Jan 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Eva MezeyBarbara Crain
Feb 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·James M WeimannHelen M Blau
Feb 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yong ZhaoEliezer Huberman
Mar 29, 2003·Journal of Hematotherapy & Stem Cell Research·Hsiao-Nan HaoWilliam D Lyman
Sep 10, 2003·Journal of Leukocyte Biology·Masataka KuwanaYasuo Ikeda
Oct 14, 2003·Nature·Manuel Alvarez-DoladoArturo Alvarez-Buylla
Nov 26, 2003·Experimental Neurology·Irene Ginis, Mahendra S Rao
May 4, 2004·Lancet·Christopher R CogleEdward W Scott
Jul 13, 2004·Lancet·C M Rice, N J Scolding
Oct 20, 2004·The Journal of Cell Biology·Katsuhisa MatsuuraIssei Komuro
Mar 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Olafur E SigurjonssonJoel C Glover
Apr 20, 2005·Journal international de bioéthique = International journal of bioethics·UNKNOWN World Medical Association General Assembly
Jan 26, 2006·Stem Cells and Development·Hiroaki KodamaMasataka Kuwana
Citations
Mar 28, 2012·Stem Cell Reviews and Reports·Yichi Zhang, Bing Huang
Feb 28, 2008·Immunology and Cell Biology·Noriyuki SetaMasataka Kuwana
Apr 11, 2013·Investigative Ophthalmology & Visual Science·Sergio CaballeroMaria B Grant
Dec 9, 2008·Blood·Haiming ChenJames R Berenson
Aug 23, 2012·PloS One·Suzanne GartnerSenthilkumar Natesan
Sep 26, 2013·PloS One·Noriyuki SetaMasataka Kuwana
Feb 20, 2014·PloS One·Michelle W Y WilliamsJohn P Fletcher
Jun 25, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jenna M Tabor-GodwinRalph Feuer
Mar 1, 2013·Nihon Rinshō Men'eki Gakkai kaishi = Japanese journal of clinical immunology·Masataka Kuwana
Jul 5, 2007·The Keio Journal of Medicine·Noriyuki Seta, Masataka Kuwana
Apr 13, 2013·Cytotechnology·Nanding WangZongren Wang
Dec 20, 2012·Clinical & Developmental Immunology·Noriyuki SetaMasataka Kuwana
May 10, 2011·Autoimmunity Reviews·Paola CiprianiRoberto Giacomelli
Apr 7, 2010·Experimental Hematology·Noriyuki Seta, Masataka Kuwana
Aug 5, 2006·Stem Cells·Masataka KuwanaYasuo Ikeda
Jul 8, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Gregor A PilzWilhelm K Aicher
Nov 8, 2011·Journal of Neuroscience Research·Hidenori HattoriMasataka Kuwana
Jul 22, 2015·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Y ZhouH Shen
Aug 19, 2015·Biochimica Et Biophysica Acta·Kazuhide Hayakawa, Eng H Lo
Jan 1, 2014·Progress in Neurobiology·Chengrui AnJun Chen
Sep 7, 2012·Biotechnology Research International·Marc-Estienne Roehrich, Giuseppe Vassalli
Feb 23, 2012·Scientific Reports·Valentina MarchettiMartin Friedlander
Sep 19, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Mahlon D JohnsonDaniele Walters
Jan 30, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Sung-Su KimChong Jai Kim
Aug 14, 2018·PloS One·Noriko OtsukiKayoko Saito
Jan 21, 2010·Neuroreport·Sandra HorschitzPatrick Schloss
Feb 15, 2019·Frontiers in Molecular Neuroscience·Alfredo BellonAnne Hosmalin
Sep 25, 2020·Stem Cell Research & Therapy·Alaknanda MishraPramod Upadhyay
Dec 5, 2017·The Journal of Clinical Investigation·Jorg DietrichDavid T Scadden
Mar 25, 2021·Experimental Cell Research·Naveen Challagundla, Reena Agrawal-Rajput
Oct 15, 2021·Biology Open·Aaron M SavageAndrew D Johnson