Neuroinvasion and Encephalitis Following Intranasal Inoculation of SARS-CoV-2 in K18-hACE2 Mice.

Viruses
Pratima KumariMukesh Kumar

Abstract

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection can cause neurological disease in humans, but little is known about the pathogenesis of SARS-CoV-2 infection in the central nervous system (CNS). Herein, using K18-hACE2 mice, we demonstrate that SARS-CoV-2 neuroinvasion and encephalitis is associated with mortality in these mice. Intranasal infection of K18-hACE2 mice with 105 plaque-forming units of SARS-CoV-2 resulted in 100% mortality by day 6 after infection. The highest virus titers in the lungs were observed on day 3 and declined on days 5 and 6 after infection. By contrast, very high levels of infectious virus were uniformly detected in the brains of all the animals on days 5 and 6. Onset of severe disease in infected mice correlated with peak viral levels in the brain. SARS-CoV-2-infected mice exhibited encephalitis hallmarks characterized by production of cytokines and chemokines, leukocyte infiltration, hemorrhage and neuronal cell death. SARS-CoV-2 was also found to productively infect cells within the nasal turbinate, eye and olfactory bulb, suggesting SARS-CoV-2 entry into the brain by this route after intranasal infection. Our data indicate that direct infection of CNS cells together with the ...Continue Reading

References

Oct 1, 1990·The Journal of Experimental Medicine·S PerlmanA Afifi
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Chris S SchaumburgThomas E Lane
Jan 16, 2010·Science·Akiko Iwasaki, Ruslan Medzhitov
Feb 15, 2015·Current Opinion in Virology·Phillip A Swanson, Dorian B McGavern
Feb 13, 2016·Cell Host & Microbe·Benjamin R tenOever
Apr 9, 2016·ACS Chemical Neuroscience·Douglas M DurrantRobyn S Klein
Apr 18, 2016·Journal of Neurochemistry·Yugandhar V Gowrisankar, Michelle A Clark
Apr 13, 2017·Virology Journal·Mukesh KumarVivek R Nerurkar
Jun 22, 2018·Journal of Virology·Mathieu DubéPierre J Talbot
Mar 3, 2020·Journal of Autoimmunity·Hussin A Rothan, Siddappa N Byrareddy
Apr 15, 2020·Therapeutic Advances in Neurological Disorders·Hai-Yang WangBing-Wei Zhang
Apr 25, 2020·Curēus·Asia FilatovPatricio S Espinosa
May 14, 2020·The New England Journal of Medicine·Victor G PuellesTobias B Huber
Jun 1, 2020·EBioMedicine·Qi ChengJianqun Gao
Jun 6, 2020·Alzheimer's Research & Therapy·Michael T HenekaRobert Brown
Jun 9, 2020·Lancet·Claus Hann von WeyhernMarcus Kremer
Jun 13, 2020·The New England Journal of Medicine·Isaac H SolomonPardis Sabeti
Jun 18, 2020·Academic Radiology·Arshed Hussain ParryMudasira Yaseen
Jun 22, 2020·Asian Pacific Journal of Allergy and Immunology·Jesada KanjanaumpornSupinda Chusakul
Jul 10, 2020·Pathogens·Hussin A RothanSiddappa N Byrareddy
Jul 30, 2020·The American Journal of Tropical Medicine and Hygiene·Gregory Brett MoreauBarbara J Mann
Aug 6, 2020·Cell Research·Bao-Zhong ZhangJian-Dong Huang
Aug 22, 2020·Polish Archives of Internal Medicine·Monika Adamczyk-SowaPaweł Sowa
Aug 26, 2020·Cellular and Molecular Neurobiology·Walter J LukiwJames M Hill
Sep 3, 2020·The EMBO Journal·Anand RamaniJay Gopalakrishnan
Oct 22, 2020·Science·Ludovico Cantuti-CastelvetriMikael Simons

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Citations

Feb 9, 2021·Brain Research·Valéry BielarzCharles Nicaise
Apr 26, 2021·Signal Transduction and Targeted Therapy·Li JiaoXiaozhong Peng
Apr 24, 2021·Frontiers in Aging Neuroscience·Isabel M Alonso-BellidoRocío Ruiz
Apr 27, 2021·ENeurologicalSci·Keith J KincaidAlexis N Simpkins
Apr 30, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Geetanjali Saini, Ritu Aneja
May 3, 2021·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Keith J Kincaid, Alexis N Simpkins
May 11, 2021·Frontiers in Pharmacology·G KanimozhiN Rajendra Prasad
Jun 10, 2021·Nature Communications·Alberto A AmarillaAlexander A Khromykh
Jun 13, 2021·Signal Transduction and Targeted Therapy·Qianqian ZhangFei Yu
Jul 3, 2021·Antioxidants·Noemí Cárdenas-RodríguezBeatriz Hernández-Ochoa

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Methods Mentioned

BETA
PCR
ELISA

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