Neuroligins Differentially Mediate Subtype-Specific Synapse Formation in Pyramidal Neurons and Interneurons

Neuroscience Bulletin
Qiang-Qiang XiaJunyu Xu

Abstract

Neuroligins (NLs) are postsynaptic cell-adhesion proteins that play important roles in synapse formation and the excitatory-inhibitory balance. They have been associated with autism in both human genetic and animal model studies, and affect synaptic connections and synaptic plasticity in several brain regions. Yet current research mainly focuses on pyramidal neurons, while the function of NLs in interneurons remains to be understood. To explore the functional difference among NLs in the subtype-specific synapse formation of both pyramidal neurons and interneurons, we performed viral-mediated shRNA knockdown of NLs in cultured rat cortical neurons and examined the synapses in the two major types of neurons. Our results showed that in both types of neurons, NL1 and NL3 were involved in excitatory synapse formation, and NL2 in GABAergic synapse formation. Interestingly, NL1 affected GABAergic synapse formation more specifically than NL3, and NL2 affected excitatory synapse density preferentially in pyramidal neurons. In summary, our results demonstrated that different NLs play distinct roles in regulating the development and balance of excitatory and inhibitory synapses in pyramidal neurons and interneurons.

References

Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·J Y SongN Brose
Aug 22, 2003·Journal of Neurophysiology·Zhanyan FuStefano Vicini
Sep 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Oliver PrangeAlaa El-Husseini
Nov 16, 2004·European Journal of Cell Biology·Frédérique VaroqueauxNils Brose
Feb 1, 2005·Science·Ben ChihPeter Scheiffele
Sep 20, 2006·Neuron·Frédérique VaroqueauxNils Brose
Sep 28, 2007·The European Journal of Neuroscience·Elaine C Budreck, Peter Scheiffele
Nov 23, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mian CaoJun Xia
Jun 26, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mrinalini HoonFrédérique Varoqueaux
Nov 6, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jay R GibsonThomas C Südhof
Feb 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jacqueline BlundellCraig M Powell
Feb 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mrinalini HoonFrédérique Varoqueaux
Sep 18, 2012·Science·Stéphane J BaudouinPeter Scheiffele
Nov 13, 2012·Nature Neuroscience·Hyung-Bae KwonBernardo L Sabatini
Sep 2, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bo Zhang, Thomas C Südhof
Jun 14, 2017·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Soham ChandaThomas C Südhof
Aug 11, 2017·Neuroscience Bulletin·Yao TianJunhai Han

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Methods Mentioned

BETA
transfection

Software Mentioned

Graphpad
AVONA

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