Neuromedin B receptor mediates neuromedin B-induced COX-2 and IL-6 expression in human primary myometrial cells.

Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research
Jingfei ChenWei-She Zhang

Abstract

The precise mechanisms that lead to parturition remain unclear. In our initial complementary DNA (cDNA) microarray experiment, we found that the neuromedin B receptor (NMBR) was differentially expressed in the human myometrium during spontaneous or oxytocin-induced labor. We have previously shown that neuromedin B (NMB) could induce interleukin 6 (IL-6) and type 2 cyclo-oxygenase enzyme (COX-2) expression in the primary human myometrial cells via nuclear factor kappa B (NF-κB) transcription factor p65 (p65) and Jun proto-oncogene, activator protein 1 (AP-1) transcription factor subunit (c-Jun). This study is aimed to investigate whether NMBR is required for NMB-induced effect. Primary myometrial cell culture was established to provide a suitable model to investigate the mechanism of NMB in labor initiation. Immunochemical staining was conducted to validate the NMBR expression in primary myometrial cells. The mRNA and protein expression of NMBR, p65, c-Jun, COX-2 and IL-6 were assessed by Quantitative Real Time PCR (RT-qPCR) and western blotting. Lentiviruses with shRNAs targeting NMBR or containing cDNA sequence of NMBR were transfected to primary myometrial cells to knockdown or overexpress NMBR. Cell death was determined by a...Continue Reading

References

May 1, 1994·European Journal of Biochemistry·T KosakaT Tanabe
Sep 1, 1997·Obstetrics and Gynecology·P C GreigJ Allen
Jun 7, 2000·Progress in Neurobiology·H Ohki-Hamazaki
Jun 25, 2002·Biology of Reproduction·Jennifer A Mitchell, Stephen J Lye
Jul 2, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·John R G ChallisJohn P Newnham
Nov 3, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Tamsin M Lindström, Phillip R Bennett
Mar 30, 2006·The Journal of Clinical Endocrinology and Metabolism·Vasso TerzidouPhillip R Bennett
Oct 3, 2006·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Suk Joo ChoiCheong Rae Roh
Jan 11, 2008·Current Opinion in Endocrinology, Diabetes, and Obesity·Nieves GonzalezRobert T Jensen
Jun 12, 2012·Mediators of Inflammation·Shirin KhanjaniPhillip R Bennett
Mar 26, 2013·Biochimica Et Biophysica Acta·Min Huan WuChih-Hsin Tang
Jan 5, 2014·Archives of Gynecology and Obstetrics·Irène HösliOlivier Irion
Feb 6, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·David A MacIntyrePhillip R Bennett

❮ Previous
Next ❯

Citations

Jan 5, 2021·Current Opinion in Endocrinology, Diabetes, and Obesity·Xiaoqun Qin, Xiangping Qu

❮ Previous
Next ❯

Methods Mentioned

BETA
cesarean section
electrophoresis
PCR
flow cytometry

Software Mentioned

GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.