Abstract
To study neuron-glial interactions, our laboratory has developed an in vitro model system that, when used with cell type-specific antisera, allows visualization of contacts between cerebellar granule neurons and astroglia. When cells were dissociated from early postnatal mouse cerebellum and plated in microcultures, the neurons aligned along glial filament protein (GFP)-containing astroglial processes. The behavior of the neurons depended on the shape of the particular astroglial cell that they contacted. Neuronal migration commonly occurred along highly elongated astroglial processes of Bergmann-like glia but was inhibited when neurons nestled among the arms of stellate astroglia. To analyze the influence of neurons on the astroglial shapes associated with neuronal migration, cerebellar granule neurons and astroglia were purified and recombined. In the absence of neurons, cerebellar astroglia assumed a flattened shape and proliferated rapidly. In the absence of astroglia, neurite outgrowth was severely impaired. When neurons were recombined with purified astroglia, astroglial proliferation slowed markedly, the shape of the astroglia transformed into complex forms, and neuron-glial interactions were seen. In tissue sections, im...Continue Reading
References
Apr 1, 1978·Experimental Cell Research·M E Hatten, R L Sidman
Jan 1, 1978·Neuroscience·E TrenknerR L Sidman
Mar 1, 1971·The Journal of Comparative Neurology·P Rakic
Nov 15, 1973·The Journal of Comparative Neurology·P Rakic, R L Sidman
Jan 1, 1974·Methods in Enzymology·M M Burger
Mar 1, 1984·Developmental Biology·P BovolentaC A Mason
Jan 1, 1984·The Journal of Cell Biology·M E HattenC A Mason
Apr 1, 1984·Brain Research·M E Hatten
May 24, 1984·Nature·A W Mudge
Sep 1, 1981·The Journal of Cell Biology·M E Hatten, R K Liem
Jan 1, 1982·Journal of Neurochemistry·R Liem
Oct 1, 1980·Developmental Biology·C LagenaurM Schachner
Aug 1, 1981·The Journal of Cell Biology·I SommerM Schachner
Jun 1, 1982·Brain Research·R H Nordlander, M Singer
Sep 1, 1982·The Journal of Comparative Neurology·J SilverJ Coughlin
Oct 15, 1981·Developmental Biology·M E Hatten, A M Francois
Jan 1, 1980·The Journal of Comparative Neurology·J Silver, R L Sidman
Citations
May 8, 2018·Experimental Neurobiology·Eun-Hye JoeSangmyun Park
Dec 18, 1995·The Journal of Comparative Neurology·K S EagleF H Gage
Jan 2, 2003·Glia·Mary Mazzanti, Philip G Haydon
Oct 16, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·B M Ajtai, M Kálmán
Dec 1, 1987·Journal of Neurochemistry·D C Larrivee, B Grafstein
Jul 23, 2005·The Cerebellum·K NguonE M Sajdel-Sulkowska
Sep 1, 2007·Developmental Neuroscience·Lisa C HuttonDavid W Walker
Apr 18, 2008·The Cerebellum·Elizabeth M Sajdel-Sulkowska
Mar 29, 2001·Molecular and Cellular Neurosciences·S KelićD E Weinstein
Feb 2, 2011·Brain Research·Mika NishimotoAkiko Furuta