PMID: 6400196Jan 1, 1984Paper

Neuron-astroglial interactions in vitro and their implications for repair of CNS injury

Central Nervous System Trauma : Journal of the American Paralysis Association
M E HattenM L Shelanski

Abstract

To study neuron-glial interactions, our laboratory has developed an in vitro model system that, when used with cell type-specific antisera, allows visualization of contacts between cerebellar granule neurons and astroglia. When cells were dissociated from early postnatal mouse cerebellum and plated in microcultures, the neurons aligned along glial filament protein (GFP)-containing astroglial processes. The behavior of the neurons depended on the shape of the particular astroglial cell that they contacted. Neuronal migration commonly occurred along highly elongated astroglial processes of Bergmann-like glia but was inhibited when neurons nestled among the arms of stellate astroglia. To analyze the influence of neurons on the astroglial shapes associated with neuronal migration, cerebellar granule neurons and astroglia were purified and recombined. In the absence of neurons, cerebellar astroglia assumed a flattened shape and proliferated rapidly. In the absence of astroglia, neurite outgrowth was severely impaired. When neurons were recombined with purified astroglia, astroglial proliferation slowed markedly, the shape of the astroglia transformed into complex forms, and neuron-glial interactions were seen. In tissue sections, im...Continue Reading

References

Jan 1, 1974·Methods in Enzymology·M M Burger
Mar 1, 1984·Developmental Biology·P BovolentaC A Mason
Jan 1, 1984·The Journal of Cell Biology·M E HattenC A Mason
Apr 1, 1984·Brain Research·M E Hatten
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Oct 15, 1981·Developmental Biology·M E Hatten, A M Francois
Jan 1, 1980·The Journal of Comparative Neurology·J Silver, R L Sidman

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Citations

May 8, 2018·Experimental Neurobiology·Eun-Hye JoeSangmyun Park
Dec 18, 1995·The Journal of Comparative Neurology·K S EagleF H Gage
Oct 16, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·B M Ajtai, M Kálmán
Dec 1, 1987·Journal of Neurochemistry·D C Larrivee, B Grafstein
Mar 29, 2001·Molecular and Cellular Neurosciences·S KelićD E Weinstein

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