Neuron-derived FGF10 ameliorates cerebral ischemia injury via inhibiting NF-κB-dependent neuroinflammation and activating PI3K/Akt survival signaling pathway in mice

Scientific Reports
Yong-Hua LiHong-Bin Yuan

Abstract

FGF10 is a member of fibroblast growth factors (FGFs). We previously showed that FGF10 protects neuron against oxygen-glucose deprivation injury in vitro; however, the effect of FGF10 in ischemic stroke in vivo is unknown. In the present study, we showed that FGF10 was mainly expressed in neurons but not astrocytes, and detected FGF10 in mouse cerebrospinal fluid. The FGF10 levels in neurons culture medium and cell lysate were much higher than those in astrocytes. FGF10 expression in brain tissue and FGF10 level in CSF were increased in mouse middle cerebral artery occlusion (MCAO) model. Administration of FGF10 into lateral cerebroventricle not only decreased MCAO-induced brain infarct volume and neurological deficit, but also reduced the number of TUNEL-positive cells and activities of Caspases. Moreover, FGF10 treatment depressed the triggered inflammatory factors (TNF-α and IL-6) and NF-κB signaling pathway, and increased phosphorylation of PI3K/Akt signaling pathway. Blockade of PI3K/Akt signaling pathway by wortmannin and Akt1/2-kinase inhibitor, partly compromised the neuroprotection of FGF10. However, blockade of PI3K/Akt signaling pathway did not impair the anti-inflammation action of FGF10. Collectively, our results d...Continue Reading

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Citations

Oct 1, 2016·BioMed Research International·Ya DingJinbo Liu
Nov 8, 2018·Médecine sciences : M/S·Wen-Juan LiuXiao Huang
Feb 3, 2019·Journal of Cellular Physiology·Dandan WangWei Wei
Apr 24, 2020·Frontiers in Pharmacology·Lian-Cheng DengJin-San Zhang
May 14, 2019·International Journal of Oncology·Yu DingCaixia Peng
Jun 26, 2021·Neurotoxicity Research·Sedigheh EskandariGholamreza Bahrami
Jul 9, 2021·Frontiers in Pharmacology·Yiqiu LiuXuqiang Nie

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Methods Mentioned

BETA
nuclear translocation

Software Mentioned

GraphPad Prism
Primer Express
Image J

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