Neuron-specific splicing of C-SRC RNA in human brain

Journal of Neuroscience Research
J M Pyper, J B Bolen

Abstract

The C-SRC, C-YES, and FYN genes encode three closely related tyrosine protein kinases that are expressed in human neural tissues. A unique form of the C-SRC gene has been demonstrated to be expressed in avian and murine brain tissues as the result of alternative splicing between the third and fourth exons. This neuronal-specific splicing event adds to the C-SRC mRNA an 18 base pair exon capable of encoding the same six amino acids in both avian and murine neural tissues. The C-YES and FYN genes share with C-SRC similar exon-intron boundaries and a high degree of amino acid sequence homology in the 3/4 exon coding region. However, potential alternative splicing of the C-YES and FYN genes in this region has not been previously investigated. In this study we have compared the expression of C-SRC, C-YES, and FYN RNAs in human lung, liver, brain, and placenta tissues and prepared cDNA clones spanning exons 3 and 4 for each of these genes from the different tissues. Sequence analysis of these cDNA clones revealed that the splicing patterns for the FYN and C-YES genes were the same among the various tissues, whereas C-SRC cDNAs isolated from brain contained 18 additional bases with the capacity to code for the same six amino acids pre...Continue Reading

References

Jan 1, 1987·Molecular and Cellular Biology·J SukegawaK Toyoshima
May 1, 1987·Molecular and Cellular Biology·C A CartwrightW Eckhart
Jan 1, 1988·Molecular and Cellular Biology·O Wiestler, G Walter
Nov 1, 1987·Molecular and Cellular Biology·J Le BeauG Walter
Dec 1, 1986·Molecular and Cellular Biology·T KawakamiK C Robbins
Sep 11, 1987·Cell·T Hunter
Jan 1, 1985·Molecular and Cellular Biology·D W FultsP F Maness
Jun 1, 1983·Molecular and Cellular Biology·P C Cotton, J S Brugge

Citations

Jul 1, 1992·Developmental Biology·J W Collett, R E Steele
Jun 1, 1991·Current Opinion in Neurobiology·K R WagnerR L Huganir
Apr 2, 1999·The European Journal of Neuroscience·R DaoudS Stamm
Aug 23, 2006·Journal of Neurochemistry·Savita KhannaChandan K Sen
May 18, 2007·Molecular Aspects of Medicine·Chandan K SenSashwati Roy
Jan 18, 2006·The Journal of Physiology·Georges DaoudJulie Lafond
Jun 16, 2007·The Journal of Biological Chemistry·Savita KhannaChandan K Sen
May 8, 2009·Biology of the Cell·Eva Sýkorová, Jirí Fajkus

Related Concepts

Brain Chemistry
DNA, Double-Stranded
Mini-Exon
Gene Amplification
Liver
Lung
Neurons
Tissue Specificity
Placenta
Proto-Oncogene Proteins

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