Neuron-Subtype-Specific Expression, Interaction Affinities, and Specificity Determinants of DIP/Dpr Cell Recognition Proteins

Filip CosmanescuLawrence Shapiro


Binding between DIP and Dpr neuronal recognition proteins has been proposed to regulate synaptic connections between lamina and medulla neurons in the Drosophila visual system. Each lamina neuron was previously shown to express many Dprs. Here, we demonstrate, by contrast, that their synaptic partners typically express one or two DIPs, with binding specificities matched to the lamina neuron-expressed Dprs. A deeper understanding of the molecular logic of DIP/Dpr interaction requires quantitative studies on the properties of these proteins. We thus generated a quantitative affinity-based DIP/Dpr interactome for all DIP/Dpr protein family members. This revealed a broad range of affinities and identified homophilic binding for some DIPs and some Dprs. These data, along with full-length ectodomain DIP/Dpr and DIP/DIP crystal structures, led to the identification of molecular determinants of DIP/Dpr specificity. This structural knowledge, along with a comprehensive set of quantitative binding affinities, provides new tools for functional studies in vivo.


Dec 14, 2002·The International Journal of Biochemistry & Cell Biology·Juha-Pekka Himanen, Dimitar B Nikolov
Oct 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·R W SPERRY
Dec 17, 2003·Current Opinion in Structural Biology·Saurabh D PatelLawrence Shapiro
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Aug 26, 2004·Developmental Biology·Noriko OkabeYoshimi Takai
Jun 28, 2006·The Journal of Cell Biology·Hideru TogashiMasatoshi Takeichi
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Oct 30, 2007·Methods in Cell Biology·James L ColeThomas M Laue
Nov 16, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Adam I FogelThomas Biederer
Feb 26, 2008·Genome Research·K Mark BushellGavin J Wright
Oct 8, 2008·Annual Review of Cell and Developmental Biology·Daisuke HattoriS Lawrence Zipursky
Oct 30, 2008·Neuron·Shuying GaoChi-Hon Lee
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Jun 26, 2009·Proceedings of the National Academy of Sciences of the United States of America·P KatsambaBarry H Honig
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Jul 6, 2010·Developmental Biology·Sujin BaoRoss L Cagan
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Oct 12, 2011·Methods in Molecular Biology·Lucia BaldiFlorian M Wurm
Apr 17, 2012·Acta Crystallographica. Section D, Biological Crystallography·Pavel V AfoninePaul D Adams
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Aug 21, 2012·Nature Structural & Molecular Biology·Oliver J HarrisonLawrence Shapiro
Oct 12, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Masahito Yamagata, Joshua R Sanes
Dec 21, 2012·Seminars in Cell & Developmental Biology·Christian Siebold, E Yvonne Jones
Jun 26, 2013·Acta Crystallographica. Section D, Biological Crystallography·Philip R Evans, Garib N Murshudov
Jul 16, 2013·Neuron·John C TuthillMichael B Reiser
Aug 9, 2013·Nature·Shin-ya TakemuraDmitri B Chklovskii
Jul 31, 2014·IUCrJ·Robbie P JoostenAnastassis Perrakis
Sep 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jeremie VendomeBarry Honig
May 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Aljoscha NernGerald M Rubin
Jul 18, 2015·Cell·Alex S MaussAlexander Borst
Sep 20, 2016·ELife·Kerry M GoodmanLawrence Shapiro

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Nov 5, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P Robin Hiesinger
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