Neuronal activity remodels the F-actin based submembrane lattice in dendrites but not axons of hippocampal neurons.

Scientific Reports
F. Lavoie-CardinalPaul De Koninck

Abstract

The nanoscale organization of the F-actin cytoskeleton in neurons comprises membrane-associated periodical rings, bundles, and longitudinal fibers. The F-actin rings have been observed predominantly in axons but only sporadically in dendrites, where fluorescence nanoscopy reveals various patterns of F-actin arranged in mixed patches. These complex dendritic F-actin patterns pose a challenge for investigating quantitatively their regulatory mechanisms. We developed here a weakly supervised deep learning segmentation approach of fluorescence nanoscopy images of F-actin in cultured hippocampal neurons. This approach enabled the quantitative assessment of F-actin remodeling, revealing the disappearance of the rings during neuronal activity in dendrites, but not in axons. The dendritic F-actin cytoskeleton of activated neurons remodeled into longitudinal fibers. We show that this activity-dependent remodeling involves [Formula: see text] and NMDA receptor-dependent mechanisms. This highly dynamic restructuring of dendritic F-actin based submembrane lattice into longitudinal fibers may serve to support activity-dependent membrane remodeling, protein trafficking and neuronal plasticity.

References

Jun 1, 1987·Brain Research Bulletin·G Lynch, M Baudry
Jan 1, 1986·Annual Review of Biochemistry·T D Pollard, J A Cooper
Jul 29, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Andy HudmonPaul De Koninck
Jan 1, 1993·IEEE Transactions on Medical Imaging·M OzkanR J Maciunas
Sep 6, 2011·Biophysical Journal·Nicolai T UrbanU Valentin Nägerl
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
May 27, 2014·Nature Methods·Gražvydas LukinavičiusKai Johnsson
Dec 24, 2014·ELife·Guisheng ZhongXiaowei Zhuang
Jul 29, 2015·The Journal of Cell Biology·Archan GangulySubhojit Roy
Apr 24, 2016·Cellular and Molecular Life Sciences : CMLS·Anaël Chazeau, Grégory Giannone
May 26, 2016·Scientific Reports·Sven C SidensteinStefan W Hell
Jun 17, 2016·Bioinformatics·Oren Z KrausBrendan J Frey
Oct 5, 2016·The Journal of Cell Biology·David AlbrechtHelge Ewers
Nov 5, 2016·PLoS Computational Biology·David A Van ValenMarkus W Covert
Nov 15, 2016·Scientific Reports·Julia BärMarina Mikhaylova
Jun 3, 2017·Frontiers in Cellular Neuroscience·Anja KonietznyMarina Mikhaylova
Jul 26, 2017·Proceedings of the National Academy of Sciences of the United States of America·Boran HanXiaowei Zhuang
Aug 12, 2017·Scientific Reports·Sajith Kecheril SadanandanCarolina Wählby
Jan 13, 2018·Frontiers in Cellular Neuroscience·Simone MortalVincent Torre
May 17, 2018·Frontiers in Molecular Neuroscience·Sreetama Basu, Raphael Lamprecht
Jun 19, 2018·Current Biology : CB·Philipp SchätzleCasper C Hoogenraad
Dec 12, 2018·Nature Communications·Audrey DurandFlavie Lavoie-Cardinal
Dec 19, 2018·Nature Methods·Thorsten FalkOlaf Ronneberger

❮ Previous
Next ❯

Citations

Jan 16, 2021·Current Opinion in Neurobiology·Christophe Leterrier
Nov 19, 2020·Brain : a Journal of Neurology·Liedewei Van de VondelDanique Beijer
Apr 17, 2021·Nature Communications·Lucas von ChamierRicardo Henriques
May 18, 2021·Computational and Structural Biotechnology Journal·Bugra Özdemir, Ralf Reski
Aug 16, 2021·Journal of Computational Neuroscience·Christian Hunley, Marcelo Marucho
Jul 7, 2021·Life Science Alliance·Beatrice Terni, Artur Llobet

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence nanoscopy
with
super-resolution microscopy
confocal microscopy
dissection

Software Mentioned

Python
NetworkX
Gly

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Molecular Biology
F D Carlson, A B Fraser
Journal of Muscle Research and Cell Motility
E H Egelman
Nature Structural Biology
E H EgelmanA McGough
© 2021 Meta ULC. All rights reserved