Neuronal adenosine A2A receptor overexpression is neuroprotective towards 3-nitropropionic acid-induced striatal toxicity: a rat model of Huntington's disease

Purinergic Signalling
M R DomeniciPatrizia Popoli

Abstract

The A2A adenosine receptor (A2AR) is widely distributed on different cellular types in the brain, where it exerts a broad spectrum of pathophysiological functions, and for which a role in different neurodegenerative diseases has been hypothesized or demonstrated. To investigate the role of neuronal A2ARs in neurodegeneration, we evaluated in vitro and in vivo the effect of the neurotoxin 3-nitropropionic acid (3-NP) in a transgenic rat strain overexpressing A2ARs under the control of the neural-specific enolase promoter (NSEA2A rats). We recorded extracellular field potentials (FP) in corticostriatal slice and found that the synaptotoxic effect of 3-NP was significantly reduced in NSEA2A rats compared with wild-type animals (WT). In addition, after exposing corticostriatal slices to 3-NP 10 mM for 2 h, we found that striatal cell viability was significantly higher in NSEA2A rats compared to control rats. These in vitro results were confirmed by in vivo experiments: daily treatment of female rats with 3-NP 10 mg/kg for 8 days induced a selective bilateral lesion in the striatum, which was significantly reduced in NSEA2A compared to WT rats. These results demonstrate that the overexpression of the A2AR selectively at the neuronal...Continue Reading

References

May 1, 1997·Neuroscience and Biobehavioral Reviews·C V BorlonganP R Sanberg
Jan 14, 1999·Journal of Neurochemistry·M R Sánchez-Carbente, L Massieu
Oct 9, 1999·Progress in Neurobiology·E BrouilletP Hantraye
Jan 5, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·G W KimP H Chan
Aug 31, 2002·Molecular Reproduction and Development·Elena PopovaMichael Bader
Sep 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Katia VaraniElena Cattaneo
Jun 24, 2004·Journal of Neuroscience Research·Servio H RamirezSanjay B Maggirwar
Apr 9, 2005·Journal of Neurochemistry·Szu-Yi ChouYijuang Chern
Jul 11, 2006·Neurobiology of Learning and Memory·Lydia Giménez-LlortMichael Bader
Feb 17, 2007·Progress in Neurobiology·Patrizia PopoliMaria P Abbracchio
Feb 20, 2007·Journal of Neuroscience Methods·William W Anderson, Graham L Collingridge
Mar 24, 2007·Progress in Neurobiology·Chiara Zuccato, Elena Cattaneo
Jun 10, 2008·Current Pharmaceutical Design·Patrizia PopoliYijuang Chern
Aug 18, 2009·Biochimica Et Biophysica Acta·Maria DamianoEmmanuel Brouillet
Dec 15, 2010·Biochimica Et Biophysica Acta·Catarina V GomesRodrigo A Cunha
Feb 22, 2011·Journal of Neuroscience Research·Shuhua MuA Reiner
Aug 31, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Valentina ChiodiMaria Rosaria Domenici
Sep 27, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sergio VisentinPatrizia Popoli
Mar 13, 2014·Nature Reviews. Neurology·Christopher A RossSarah J Tabrizi
Jul 2, 2014·Frontiers in Psychiatry·Joana E CoelhoLuísa V Lopes
Sep 2, 2014·International Review of Neurobiology·Chien-fei Lee, Yijuang Chern
Dec 9, 2014·Expert Opinion on Therapeutic Targets·Kjell FuxeDasiel O Borroto-Escuela
Mar 15, 2015·Clinical Neuropharmacology·Tomoyoshi KondoUNKNOWN Japanese Istradefylline Study Group

❮ Previous
Next ❯

Citations

May 17, 2019·Journal of Neurochemistry·Amy I Smith-DijakLynn A Raymond

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
biopsies

Software Mentioned

WinLTP
GraphPad
GraphPad Prism
Image J

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.