Neuronal Cdc2-like protein kinase (Cdk5/p25) is associated with protein phosphatase 1 and phosphorylates inhibitor-2

The Journal of Biological Chemistry
A Agarwal-Mawal, Hemant K Paudel

Abstract

Protein phosphatase 1 (PP1) is complexed with inhibitor 2 (I-2) in the cytosol. In rabbit muscle extract PP1.I-2 is activated upon preincubation with ATP/Mg. This activation is caused by phosphorylation of I-2 on Thr(72) by glycogen synthase kinase 3 (GSK3). We have found that PP1.I-2 in bovine brain extract is also activated upon preincubation with ATP/Mg. However, blocking GSK3 action by LiCl inhibited only approximately 29% of PP1 activity and indicated that GSK3 is not the sole PP1.I-2 activator in the brain. When bovine brain extract was analyzed by gel filtration PP1.I-2 and neuronal Cdc2-like protein kinase (NCLK), a heterodimer of Cdk5 and the regulatory p25 subunit, co-eluted as a approximately 450-kDa size species. The NCLK from the eluted column fractions bound to PP1-specific microcystin-Sepharose and glutathione S-transferase (GST)-I-2-coated glutathione-agarose beads. Similarly, PP1 from the eluted column fractions was pulled down with GST-Cdk5-coated glutathione-agarose beads. In vitro, NCLK phosphorylated I-2 on Thr(72) and activated PP1.I-2 in an ATP/Mg-dependent manner. NCLK bound to PP1 through its Cdk5 subunit and the PP1 binding region was localized to Cdk5 residues 28-41. Our data demonstrate that in brain...Continue Reading

References

Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C P ChanE H Fischer
Jun 15, 1983·European Journal of Biochemistry·T J ResinkP Cohen
May 1, 1995·Neurobiology of Aging·J LewJ H Wang
Dec 19, 1994·FEBS Letters·M R HellmichJ F Battey
Feb 15, 1995·Biochemical and Biophysical Research Communications·F Puntoni, E Villa-Moruzzi
Sep 1, 1994·European Journal of Biochemistry·J VeselýS Leclerc
Jun 17, 1993·Nature·H L De BondtS H Kim
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·P S Klein, D A Melton
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·T OhshimaA B Kulkarni
Sep 4, 1998·Frontiers in Bioscience : a Journal and Virtual Library·C J Oliver, S Shenolikar
Mar 13, 1999·The Journal of Biological Chemistry·H B HuangA C Nairn
Mar 17, 1999·Frontiers in Bioscience : a Journal and Virtual Library·E Y LeeE R Belmonte
Jun 11, 1999·Biochemical and Biophysical Research Communications·K C ChouR L Heinrikson
Apr 6, 2000·The Journal of Biological Chemistry·K SobueH K Paudel
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·K X Huang, H K Paudel

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Citations

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Nov 6, 2013·The Journal of Cell Biology·Hailong HouHouhui Xia
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Jul 12, 2011·Annual Review of Cell and Developmental Biology·Susan C Su, Li-Huei Tsai
Oct 20, 2012·Journal of Molecular Neuroscience : MN·Sara L C EstevesEdgar F da Cruz e Silva
Feb 16, 2005·Proteomics·Frank GillardonChristopher Gerner
Apr 20, 2005·Journal of Cellular Biochemistry·Frank GillardonBernd Sommer
Mar 11, 2004·Journal of Neurochemistry·Shirley B Shelton, Gail V W Johnson
Sep 14, 2007·Molecular Microbiology·Dongqing HuangBrenda Andrews
Oct 13, 2006·Journal of Neurochemistry·Marco AngeloK Peter Giese
May 12, 2004·Free Radical Biology & Medicine·Cristian A ZambranoChristian González-Billault
Nov 1, 2005·Archives of Biochemistry and Biophysics·Hua-Shan HuangErnest Y C Lee
Apr 10, 2003·Biochemical and Biophysical Research Communications·Shijian ChuThomas J Ferro
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Sep 17, 2014·Frontiers in Aging Neuroscience·John F Castro-AlvarezGloria P Cardona-Gómez
Sep 11, 2002·The Journal of Biological Chemistry·Masumi EtoDavid L Brautigan
Jun 29, 2006·The Journal of Biological Chemistry·Florian PlattnerK Peter Giese
Jan 29, 2002·The Journal of Biological Chemistry·Wei SunHemant K Paudel
Oct 31, 2002·The Journal of Biological Chemistry·Yves S H TanJohn F Cannon
Jan 13, 2004·Physiological Reviews·Hugo Ceulemans, Mathieu Bollen
Sep 25, 2002·The Journal of Biological Chemistry·Ryan T Terry-LorenzoShirish Shenolikar
Nov 13, 2004·Journal of Cell Science·Gail V W Johnson, William H Stoothoff
May 1, 2007·Biochemical and Biophysical Research Communications·S Andrew BullockHugh C Hemmings

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