Neuronal effects of 4-t-Butylcatechol: a model for catechol-containing antioxidants

Toxicology and Applied Pharmacology
Yi-Ching LoLeo T Burka

Abstract

Many herbal medicines and dietary supplements sold as aids to improve memory or treat neurodegenerative diseases or have other favorable effects on the CNS contain a catechol or similar 1,2-dihydroxy aromatic moiety in their structure. As an approach to isolate and examine the neuroprotective properties of catechols, a simple catechol 4-t-Butylcatechol (TBC) has been used as a model. In this study, we investigated the effects of TBC on lipopolysaccharide (LPS)-activated microglial-induced neurotoxicity by using the in vitro model of coculture murine microglial-like cell line HAPI with the neuronal-like human neuroblastoma cell line SH-SY5Y. We also examined the effects of TBC on 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in human dopaminergic neuroblastoma SH-SY5Y cells. TBC at concentrations from 0.1-10 microM had no toxic effect on HAPI cells and SH-SY5Y cells, and it inhibited LPS (100 ng/ml)-induced increases of superoxide, intracellular ROS, gp91(Phox), iNOS and a decrease of HO-1 in HAPI cells. Under coculture condition, TBC significantly reduced LPS-activated microglia-induced dopaminergic SH-SY5Y cells death. Moreover, TBC (0.1-10 microM) inhibited 6-OHDA-induced increases of intracellular ROS, iNOS, nNOS, and a d...Continue Reading

Citations

Apr 3, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Yu-Tzu ShihYi-Ching Lo
May 19, 2009·Environmental and Molecular Mutagenesis·George BarretoLisandro Diego Alvarez-Giraldez
Jul 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yih-Fung ChenYi-Ching Lo
Aug 12, 2018·Journal of Neuroscience Research·Vittorio CalabreseEdward J Calabrese
Jan 12, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Nina VorobjevaBoris Chernyak

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