PMID: 2746241Aug 1, 1989Paper

Neuronal glutamine utilization: pathways of nitrogen transfer studied with [15N]glutamine

Journal of Neurochemistry
M YudkoffM Erecińska


Gas chromatography-mass spectrometry was used to evaluate the metabolism of [15N]glutamine in isolated rat brain synaptosomes. In the presence of 0.5 mM glutamine, synaptosomes accumulated this amino acid to a level of 25-35 nmol/mg protein at an initial rate greater than 9 nmol/min/mg of protein. The metabolism of [15N]glutamine generated 15N-labelled glutamate, aspartate, and gamma-aminobutyric acid (GABA). An efflux of both [15N]glutamate and [15N]aspartate from synaptosomes to the medium was observed. Enrichment of 15N in alanine could not be detected because of a limited pool size. Elimination of glucose from the incubation medium substantially increased the rate and amount of [15N]aspartate formed. It is concluded that: (1) With 0.5 mM external glutamine, the glutaminase reaction, and not glutamine transport, determines the rate of metabolism of this amino acid. (2) The primary route of glutamine catabolism involves aspartate aminotransferase which generates 2-oxoglutarate, a substrate for the tricarboxylic acid cycle. This reaction is greatly accelerated by the omission of glucose. (3) Glutamine has preferred access to a population of synaptosomes or to a synaptosomal compartment that generates GABA. (4) Synaptosomes mai...Continue Reading


Mar 25, 1977·Science·A Martinez-HernandezM D Norenberg
Feb 2, 1979·Brain Research·M D Norenberg, A Martinez-Hernandez
Mar 1, 1979·Journal of Neurochemistry·E G McGeer, P L McGeer
Jun 1, 1978·Journal of Neurochemistry·H F BradfordA J Thomas
Feb 1, 1979·Journal of Neurochemistry·C T WeilerA Hamberger
May 15, 1976·The Biochemical Journal·S C DennisJ B Clark
Jun 25, 1976·Brain Research·H F Bradford, H K Ward
Feb 15, 1986·The Biochemical Journal·M YudkoffD Pleasure
Dec 1, 1987·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M B Robinson, J T Coyle
Sep 1, 1988·Journal of Neurochemistry·M YudkoffD Pleasure
Sep 1, 1988·Journal of Neurochemistry·M ErecińskaM Yudkoff
Nov 28, 1986·Science·D K Srivastava, S A Bernhard
Oct 1, 1974·Journal of Neurochemistry·R J Baldessarini, C Yorke
Jul 1, 1972·Journal of Neurochemistry·L R GjessingO Sjaastad
Dec 1, 1971·The American Journal of Physiology·W H Oldendorf
Nov 1, 1969·Journal of Neurochemistry·H F Bradford, A J Thomas
Jun 1, 1969·Journal of Neurochemistry·H Weil-Malherbe
Jan 1, 1966·The Biochemical Journal·J L Mangan, V P Whittaker
Jun 1, 1981·Journal of Neurochemistry·E Kvamme, B E Olsen
Jul 22, 1982·Brain Research·N RamaharobandroJ Mark
Mar 1, 1983·Journal of Neurochemistry·H K WardH F Bradford
Sep 1, 1983·Journal of Neurochemistry·C M ThankiH F Bradford
Jan 1, 1984·Journal of Neurochemistry·F Fonnum
Sep 1, 1963·The Biochemical Journal·R J HASLAM, H A KREBS
Feb 1, 1965·Journal of Neurochemistry·R BALAZS
Jun 1, 1960·The Biochemical Journal·H A KREBS, D BELLAMY

❮ Previous
Next ❯


Dec 18, 2001·Journal of Neuroscience Research·M YudkoffI Nissim
Oct 1, 1992·Journal of Neuroscience Research·L HertzA Schousboe
Jan 1, 1990·Molecular and Chemical Neuropathology·I Torgner, E Kvamme
Mar 13, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·O A PetroffJ W Prichard
Jan 1, 1990·Progress in Neurobiology·M Erecińska, I A Silver
Jul 23, 2003·Experimental Eye Research·B V BuiMichael Kalloniatis
Nov 21, 2002·Neurochemistry International·Hong QuUrsula Sonnewald
Mar 25, 2000·Neurochemistry International·M YudkoffI Nissim
Nov 6, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xiaodong BaoElias K Michaelis
Jul 1, 1991·Journal of Neurochemistry·M YudkoffM Erecińska
Jun 1, 1990·Journal of Neurochemistry·M ErecińskaM Yudkoff
Oct 13, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Elisabeth OlstadUrsula Sonnewald
Aug 4, 2015·Neurochemical Research·Thomas M JeitnerArthur J L Cooper
Sep 10, 2004·The European Journal of Neuroscience·Bang V BuiMichael Kalloniatis
Jul 15, 2006·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·William S A Brusilow
Jun 18, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Michaela C HohnholtHelle S Waagepetersen
Jun 15, 2010·Glia·Tore Wergeland MeisingsetUrsula Sonnewald
Aug 26, 2009·Journal of Neurochemistry·Bang V BuiMichael Kalloniatis
Mar 29, 2000·The Journal of Nutrition·Y Daikhin, M Yudkoff
Jun 22, 2000·The Journal of Comparative Neurology·J LevensonA Eskin
Sep 29, 1999·Journal of Neurochemistry·H S WaagepetersenA Schousboe
Nov 12, 1996·Biochimica Et Biophysica Acta·M ErecińskaI A Silver
May 20, 2004·Journal of Inorganic Biochemistry·Lidia Struzyńska, Grzegorz Sulkowski

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.