Neuronal mechanisms of active (rapid eye movement) sleep induced by microinjections of hypocretin into the nucleus pontis oralis of the cat

Neuroscience
M-C Xi, Michael H Chase

Abstract

Hypocretinergic (orexinergic) neurons in the hypothalamus project to the nucleus pontis oralis, a nucleus which plays a crucial role in the generation of active (rapid eye movement) sleep. We recently reported that the microinjection of hypocretin into the nucleus pontis oralis of chronically-instrumented, unanesthetized cats induces a behavioral state that is comparable to naturally-occurring active sleep. The present study examined the intracellular signaling pathways underlying the active sleep-inducing effects of hypocretin. Accordingly, hypocretin-1, a protein kinase C inhibitor and a protein kinase A inhibitor were injected into the nucleus pontis oralis in selected combinations in order to determine their effects on sleep and waking states of chronically instrumented, unanesthetized cats. Microinjections of hypocretin-1 into the nucleus pontis oralis elicited active sleep with a short latency. However, a pre-injection of bisindolylmaleimide-I, a protein kinase C-specific inhibitor, completely blocked the active sleep-inducing effects of hypocretin-1. The combined injection of bisindolylmaleimide-I and hypocretin-1 significantly increased the latency to active sleep induced by hypocretin-1; it also abolished the increase ...Continue Reading

References

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Citations

Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Lior AppelbaumPhilippe Mourrain
Aug 16, 2008·The European Journal of Neuroscience·Elena Moreno-BalandránIsabel de Andrés
Apr 9, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Berta García-GarcíaMargarita L Rodrigo-Angulo

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