Neuronal NOS gene expression in gastrointestinal myenteric neurons and smooth muscle cells

The American Journal of Physiology
S ChakderSatish Rattan

Abstract

Nitric oxide synthase (NOS) has been characterized in different tissues, and its localization has been suggested in different neuronal tissues, including the myenteric neurons and other nonneuronal cells. The present study examined the distribution of the neuronal NOS (nNOS) mRNA in different tissues of the opossum gastrointestinal tract, internal anal sphincter (IAS) smooth muscle cells, and myenteric neurons using slot-blot and Northern blot hybridization techniques with a specific rat brain nNOS cDNA probe. Significant levels of nNOS gene expression were found in both smooth muscle cells and myenteric neurons of the opossum IAS. This finding was confirmed by reverse transcriptase-polymerase chain reaction analysis of the RNA obtained from cultured opossum IAS smooth muscle cells and myenteric neurons and also from human intestinal smooth muscle and neuroblastoma cell lines. Pyloric sphincter had the highest level of nNOS gene expression compared with other gastrointestinal tissues. There was no significant difference in the nNOS gene expression between other sphincteric and nonsphincteric tissues examined. The present study shows the presence of nNOS gene expression in both neurons and smooth muscle cells. The higher levels ...Continue Reading

References

Jul 12, 1979·Nature·J AlumetsR Uddman
Dec 1, 1992·Gastroenterology·M E Stark, J H Szurszewski
Jan 1, 1992·Neuron·D S Bredt, S H Snyder
Aug 20, 1992·The New England Journal of Medicine·J M VanderwindenM H De Laet
Aug 6, 1991·European Journal of Pharmacology·I FlemingJ C Stoclet
Apr 1, 1988·The Journal of Clinical Investigation·S Nurko, S Rattan
Mar 1, 1994·The Biochemical Journal·R G Knowles, S Moncada
Apr 1, 1994·The American Journal of Physiology·I BerezinE E Daniel
Jul 1, 1996·The Journal of Clinical Investigation·H MashimoR K Goyal

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Citations

Jul 17, 1999·The American Journal of Physiology·Y P FanS Rattan
Feb 24, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·S PremaratneM L Schubert
Jan 5, 2002·The Journal of Physiology·Joëlle M C DickRomain A Lefebvre
Jul 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Rajinder N PuriSatish Rattan
Aug 13, 2008·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·B A PatelX Bian
Mar 23, 2018·The Bulletin of Tokyo Dental College·Ryuta KikuchiHiroki Watanabe
Aug 28, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Satish RattanMárcio A F De Godoy
Sep 12, 2003·Experimental Biology and Medicine·Satish RattanYa-Ping Fan
Jan 24, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Dieter SaurHans-Dieter Allescher
Apr 20, 2005·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·S Rattan
Dec 22, 1999·The Journal of Physiology·S R BartlettR Poston
Aug 13, 1999·The American Journal of Physiology·C D KimH Mashimo
Feb 25, 2021·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Arjun SinghSatish Rattan
Nov 14, 1998·The American Journal of Physiology·A HuberH D Allescher
Feb 28, 2004·Gastroenterology·Vijay ShahGianrico Farrugia

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