Neuronal precursors in the postnatal mouse cerebellum are fully committed cells: evidence from heterochronic transplantations

The European Journal of Neuroscience
A JankovskiC Sotelo

Abstract

Neural progenitors are thought to be multipotent cells whose adult phenotype is determined by extrinsic influences acting during and immediately after their last mitosis. To test this hypothesis, postnatal cerebellar precursor cells were placed in the heterochronic cellular environment of the embryonic mouse cerebellar anlage and the resulting phenotypes were determined. To identify the cells arising from postnatal precursors, tissue fragments taken from 3- to 8-day-old cerebellum of several transgenic mouse lines (each expressing the lacZ reporter gene in different sets of neuronal populations) were mixed with fragments taken from the wild-type cerebellar primordium of 12- or 13-day-old embryos. The fragments were dissociated and grafted into the cerebellum of adult mice. The phenotype acquired by postnatal precursors in the mixed grafts was determined by their morphology and ultrastructural features and by the expression of specific markers. Only two adult phenotypes were generated by these precursors: granule cells and molecular layer interneurons. Most granule cells were well integrated in the trilaminated cortex of the graft, being positioned in their proper layer both during development and after complete maturation. By c...Continue Reading

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Citations

Jul 24, 2010·Cell and Tissue Research·Changzheng ZhangTianmiao Hua
Mar 13, 2012·Development, Growth & Differentiation·Mikio Hoshino
Mar 3, 2007·The European Journal of Neuroscience·Konstantinos Ampatzis, Catherine R Dermon
Apr 14, 2010·The European Journal of Neuroscience·Constantino Sotelo
Jun 23, 2004·Molecular and Cellular Neurosciences·Ana Milosevic, James E Goldman
Feb 17, 2009·Developmental Biology·Piercesare GrimaldiMarion Wassef
Oct 4, 2007·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Barbara Carletti, Ferdinando Rossi
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Sep 25, 2002·The Journal of Comparative Neurology·Ana Milosevic, James E Goldman
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Jul 5, 2006·The European Journal of Neuroscience·Piercesare Grimaldi, Ferdinando Rossi
Jul 7, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·A J Kalyani, M S Rao
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Aug 23, 2005·Brain Research. Brain Research Reviews·Piercesare GrimaldiFerdinando Rossi
Oct 2, 2004·Journal of Neurobiology·H BecqA Represa

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